We narrowed to 13,665 results for: crispr cas9

1. OgeuIscB CAG RNA

   Plasmid

   #222867

   Purpose

   This plasmid codes for the guide of the OgeuIscB with a CAG target

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   OgeuIscB omega RNA with a (CAG)6 target sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Oct. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSc2

   Plasmid

   #80437

   Purpose

   Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

   Depositor

   Ervin Welker

   Article

   Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

   Inserts

   sp Cas9 gRNA
   eGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKSE401

   Plasmid

   #62202

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   35S and AtU6-26p

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

   Plasmid

   #83919

   Purpose

   Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   DsRed-Express2
   Bsr
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A

   Promoter

   hUbC

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. IL1RN gRNA4

   Plasmid

   #113133

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, Synthetic Biology, and TALEN

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-NLS-AcrIIA4

   Plasmid

   #113038

   Purpose

   AAV vector for expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SV40 NLS-AcrIIA4
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)

   Plasmid

   #120297

   Purpose

   AAV vector for expression of AcrIIA4 (no miR binding sites, control vector)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIA4
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV CMV-driven AcrIIA4-2xmiR-122 target sites

   Plasmid

   #120298

   Purpose

   AAV vector for expression of AcrIIA4 with two miR-122 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIA4-2xmiR-122 binding sites
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

9. pEJS540-pCSDest2-AcrIIC4Hpa

   Plasmid

   #113434

   Purpose

   Expresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   Type II-C anti-CRISPR
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS542-pCSDest2-AcrIIC5Smu

    Plasmid

    #113435

    Purpose

    Expresses type II-C anti-CRISPR protein from S. muelleri in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV CMV-driven AcrIIA4-2xmiR-1 target sites

    Plasmid

    #120299

    Purpose

    AAV vector for expression of AcrIIA4 with two miR-1 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIA4-2xmiR-1 binding sites
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV CMV-driven AcrIIC1-2xmiR-122 target sites

    Plasmid

    #120302

    Purpose

    AAV vector for expression of AcrIIC1 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIC1-2xmiR-122 binding sites
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV CMV-driven AcrIIC3-2xmiR-122 target sites

    Plasmid

    #120303

    Purpose

    AAV vector for expression of AcrIIC3 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIC3-2xmiR-122 binding sites
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

14. FD1

    Bacterial Strain

    #125258

    Purpose

    Derivative of MG1655 used for the screening of clones able to survive the bad seed toxicity of dCas9, while still efficiently silencing the expression of mcherry

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Bacterial Resistance

    None

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

15. pRARB.1.0-gDNA

    Plasmid

    #132471

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    RARB (RARB Human)
    

    Use

    CRISPR

    Available Since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX458_ZNF3_1

    Plasmid

    #72363

    Purpose

    Encodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Insert

    ZNF3 (ZNF3 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX458_ZNF3_2

    Plasmid

    #72364

    Purpose

    Encodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Insert

    ZNF3 (ZNF3 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSC5

    Plasmid

    #104779

    Purpose

    pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXPR_502

    Plasmid

    #96923

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF

    Depositor

    David Root, John Doench

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMpGE014

    Plasmid

    #108682

    Purpose

    All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)

    Depositor

    Takayuki Kohchi, Shigeo Sugano

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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