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Addgene

We narrowed to 13,844 results for: cas9 genes

Showing: 11721 - 11760 of 13844 results
  1. pORFE0003

    Plasmid
    #112072
    Purpose
    AtDeadCAS9 D10A H840A module CDS1
    Insert
    AtDeadCAS9 D10A H840A
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pORFE1052

    Plasmid
    #112078
    Purpose
    Overexpression of the AtCAS9 nickase in plant under 2x35S promoter
    Insert
    AtCAS9 D10A
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Promoter
    2x35S
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pORFE0014

    Plasmid
    #112076
    Purpose
    AtCAS9 nickase D10A module CDS1ns
    Insert
    AtCAS9 D10A without stop codon
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pSmart-Nm-sgRNA-BbsI

    Plasmid
    #49157
    Purpose
    Plasmid for cloning spacer into sgRNA for NmCas9
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. pORFE0005

    Plasmid
    #112073
    Purpose
    AtDeadCAS9 D10A H840A without stop codon module CDS1ns
    Insert
    AtDeadCAS9 D10A H840A without stop codon
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pSB700-ACTC1-Puro

    Plasmid
    #128324
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Insert
    gRNA against human ACTC1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pSB700-mouse-TTN-Cerulean

    Plasmid
    #128325
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Insert
    gRNA against mouse TTN for activation
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pSB700-TTN-Puro

    Plasmid
    #128323
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Insert
    gRNA against human TTN for activation
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. pYTP MYRF sg1

    Plasmid
    #88857
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg1 cloned into BbsI site
    Depositor
    Insert
    dCas9VP160 and MYRF sg1 gRNA (MYRF Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pYTP MYRF sg2

    Plasmid
    #88858
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg2 cloned into BbsI site
    Depositor
    Insert
    dCas9VP160 and MYRF sg2 gRNA (MYRF Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. pYTP MYRF sg3

    Plasmid
    #88859
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg3 cloned into BbsI site
    Depositor
    Insert
    dCas9VP160 and MYRF sg3 gRNA (MYRF Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. pYTP MYRF sg4

    Plasmid
    #88860
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg4 cloned into BbsI site
    Depositor
    Insert
    dCas9VP160 and MYRF sg4 gRNA (MYRF Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pYTP MYRF sg5

    Plasmid
    #88861
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg5 cloned into BbsI site
    Depositor
    Insert
    dCas9VP160 and MYRF sg5 gRNA (MYRF Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  15. pYTP MYRF sg6

    Plasmid
    #88862
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg6 cloned into BbsI site
    Depositor
    Insert
    dCas9VP160 and MYRF sg6 gRNA (MYRF Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  16. pSB700-mouse-ACTC1-Puro

    Plasmid
    #128326
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Insert
    gRNA against mouse ACTC1 for activation (Actc1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  17. pMH220

    Plasmid
    #122836
    Purpose
    dCas9 with GreenGate B-C flanking sequences
    Depositor
    Insert
    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    Use
    Golden gate compatible cloning vector
    Tags
    Expression
    Mutation
    Contains D10A and H840A mutations
    Promoter
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
  18. pSJX022

    Plasmid
    #232325
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and cat
    Expression
    Bacterial
    Mutation
    Promoter
    PJ23119 and Pvan
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  19. pSJX016

    Plasmid
    #232324
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    PJ23119 and Pvan
    Available since
    June 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  20. pIF1079

    Plasmid
    #237445
    Purpose
    Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.
    Depositor
    Inserts
    SpCas9
    Efe1-RT
    EMX1 sgRNA
    Efe1-ncRNA targeting EMX1
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CAG, H1, and U6
    Available since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  21. pSJX024

    Plasmid
    #232327
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and cat
    Expression
    Bacterial
    Mutation
    Promoter
    PJ23119 and Pxyl
    Available since
    June 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  22. pSJX067

    Plasmid
    #232328
    Purpose
    Genome editing in Agrobacterium fabrum
    Depositor
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    PJ23119 and Pvan
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  23. pLCKO2::TKOv3

    Pooled Library
    #125517
    Purpose
    Human CRISPR lentiviral library containing 70,948 guides (4-5 guides/gene) targeting 18,053 human protein-coding genes, in a non-Cas9 containing backbone
    Depositor
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 11721 - 11760 of 13844 results