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pENTR223-BBRF1

Plasmid

#195826

Purpose

Gateway Entry Clone of Epstein-Barr virus BBRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BBRF1

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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sAB-MBP

Plasmid

#204736

Purpose

synthetic antigen-binding fragment specifically recognizing bacteria maltose-binding protein

Depositor

Minglei Zhao

Article

Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d

Insert

synthetic antigen-binding fragment recognizing bacteria maltose-binding protein

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSUPERretro-Sirt7 shRNA2

Plasmid

#53145

Purpose

shRNAi vector for Sirt7

Depositor

Katrin Chua

Article

Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

Insert

Sirt7

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1 RNA polymerase III

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
pSUPERretro-Sirt7 shRNA1

Plasmid

#53144

Purpose

shRNAi vector for Sirt7

Depositor

Katrin Chua

Article

Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

Insert

Sirt7

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1 RNA polymerase III

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
5'-BirA-FLAG GFP minimal CMV pCDNA5

Plasmid

#212087

Purpose

LR vector for integration of GFP into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

GFP

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag

Plasmid

#132409

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRanCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H147A; H1044A

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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PXL

Plasmid

#75349

Purpose

PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.

Depositor

Bryan Luikart

Article

Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Sa-SauriCas9

Plasmid

#135967

Purpose

Expresses Sa-SauriCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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P023-mPB-synHEK3-BsaI

Plasmid

#239975

Purpose

PiggyBac plasmid for synthetic DNA Tape (HEK3 locus)

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBRC_double_attB_GFP_donor

Plasmid

#169692

Purpose

cargo vector for the phiC31 system with Psur5:GFP, cb_unc-119, and Gateway R1R2 site (for gene of interest insertion)

Depositor

John Wang

Article

Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

Type

Empty backbone

Expression

Worm

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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Pinducer20-dCas9-SunTag-2A-BFP

Plasmid

#115779

Purpose

targeted DNA methylation tool

Depositor

Margaret Goodell

Article

Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

Insert

dCas9-SunTag-2A-BFP

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-nArgBP2

Plasmid

#74514

Purpose

Express GFP-nArgBP2 fusion protein in mammalian cells

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

nArgBP2 (Sorbs2 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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LLP777_SnoopCatcher-p65HSF1-CO

Plasmid

#211770

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SnoopCatcher-p65HSF1

Tags

2xOLLAS

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect p65HSF…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-UnaG-P2A-FusionRed

Plasmid

#191109

Purpose

Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

UnaG

Use

AAV

Tags

FusionRed

Expression

Mammalian

Mutation

See depositor comments below

Promoter

chicken β-actin promoter

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19/Mito-mChe

Plasmid

#183167

Purpose

Construct for transient expression of mitochondrion-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

Mito-mCherry

Tags

OsCOX11, Oryza sativa cytochrome c oxidase assemb…

Expression

Plant

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR223-GFP

Plasmid

#198589

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

GFP

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-SNAP

Plasmid

#174879

Purpose

PiggyBac transposon vector for expression of NLS-SNAP

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

NLS-SNAP

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-oScarlet-WPRE-BC1-pA

Plasmid

#220765

Purpose

Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

Insert

oScarlert-WPRE-BC1

Use

AAV

Promoter

CAG

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-oScarlet-WPRE-BC2-pA

Plasmid

#220766

Purpose

Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

Insert

oScarlert-WPRE-BC2

Use

AAV

Promoter

CAG

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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FUGW-UBC-EGFP-2A-BoNT-B (LC)

Plasmid

#185722

Purpose

Lentiviral expression of GFP and botulinum neurotoxin type B light chain from UbC promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

botulinum neurotoxin type B light chain

Use

Lentiviral

Expression

Mammalian

Promoter

hUbC

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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