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PiggyBac dCas9-VPR

Plasmid

#191269

Purpose

piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Inserts

VPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-ZFN-AAVS1

Plasmid

#89707

Purpose

Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

Depositor

Dmitriy Mazurov

Article

Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

Insert

ZFN-AAVS1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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BirA in pET28a (w400-2)

Plasmid

#26624

Depositor

Eric Campeau

Insert

BirA

Tags

His

Expression

Bacterial

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

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pKM496 (deletions) Zeo

Plasmid

#109301

Purpose

ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRH030

Plasmid

#159633

Purpose

CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

Depositor

Robert Hertel

Article

Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

Type

Empty backbone

Use

CRISPR; Shuttle vector

Promoter

Constitutive tracrRNA

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET-hisT4DNALig

Plasmid

#216671

Purpose

Expresses his-tagged T4 DNA ligase in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

T4 DNA Ligase (30 T4 phage)

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVone-AAV9-CMV-eGFP

Plasmid

#230928

Purpose

pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.

Depositor

Qizhao Wang

Article

Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

Insert

eGFP

Use

AAV

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MLM3613

Plasmid

#42251

Purpose

Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

Streptococcus pyogenes Cas9

Use

CRISPR; Zebrafish expression

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-MCS-GFP-SV-puro

Plasmid

#73582

Purpose

Third generation lentiviral vector expressing GFP and puromycin

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

GFP tag

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-hLC3B-pcDNA3.1

Plasmid

#40827

Depositor

David Rubinsztein

Article

Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

Insert

LC3B (MAP1LC3B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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