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pGEX-C/EBPbZIP (pGEX-bZIPa)

Plasmid

#12435

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

68 a.a. fragment of C/EBP alpha (CEBPA Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pGP-AAV-CAG-flex-iGABASnFR2-WPRE

Plasmid

#218880

Purpose

AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Insert

iGABASnFR2

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

CAG

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 blast tBID-flag

Plasmid

#211533

Purpose

Expresses truncated BID upon doxycycline addition to trigger apoptosis

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

tBID-flag (BID Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Truncated (CASP8) form (p15)

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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BSD-CryC

Plasmid

#209672

Purpose

Expresses CRY2 fused with Split-TurboID-C and mCherry in mammalian cells

Depositor

Sanjeevi Sivasankar

Article

Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

Insert

CRY2 (CRY2 Mustard Weed)

Use

Lentiviral

Tags

Split-TurboID-C and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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CibN

Plasmid

#209671

Purpose

Expresses CIB1 fused with Split-TurboID-N and pmGFP in mammalian cells

Depositor

Sanjeevi Sivasankar

Article

Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Split-TurboID-N and pmEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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eDrFGFR1

Plasmid

#183994

Purpose

Optically controlled eDrFGFR1 expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrFGFR1 (FGFR1 Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRGEN-CMV-VPR-L1-CjCas9 V-D

Plasmid

#169913

Purpose

Expression of a VPR-dCjCas9 fusion protein to activate gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

dCjCas9

Use

CRISPR

Tags

2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

Expression

Mammalian

Mutation

D8A, H559A in cjCas9

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159845

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMC059-HisMS2_PLP_NucPhos_pac

Plasmid

#155039

Purpose

Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein gene

Depositor

Paul Freemont

Article

Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

Inserts

Maturation Protein
Coat Protein Dimer
Nucleocapsid Phosphoprotein

Expression

Bacterial

Mutation

Remove TypeIIs Restriction Sites

Promoter

T7

Available Since

Sept. 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75236

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Plxna3-Fc-His

Plasmid

#72124

Purpose

Expresses the extracellular region of the PlexinA3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna3 (Plxna3 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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Sema4d-Fc-His

Plasmid

#72157

Purpose

Expresses the extracellular region of the Sema4D protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4d (Sema4d Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pEYFPC1-ECFP/GgVcl 1-419 control 1 FRET

Plasmid

#46277

Depositor

Susan Craig

Article

Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

Insert

ECFP + Vcl 1-419 (VCL Chicken)

Tags

EYFP

Expression

Mammalian

Mutation

aa 1-419

Promoter

CMV

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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2xFRB-myc

Plasmid

#20227

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xFRB (MTOR Human)

Tags

myc

Expression

Mammalian

Mutation

1 FRB copy has a T2098L point mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDisplay FLIPE-100u

Plasmid

#13547

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

S118W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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Venus1-Y705F-STAT3

Plasmid

#123172

Purpose

Expresses Y705F STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

Y705F substitution

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Venus2-WTSTAT3

Plasmid

#123165

Purpose

Expresses wild type STAT3 fused to the 158-238 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)

Tags

Venus2 (aa 158-238) for bimolecular fluorescence …

Expression

Mammalian

Mutation

none

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-Y419F

Plasmid

#140316

Purpose

Expresses HA-tagged human Src-Y419F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

Y419F

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-His-hCE

Plasmid

#112710

Purpose

Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNGTT (RNGTT Human)

Tags

6xHis and Stop codons placed after RNGTT sequence…

Expression

Bacterial

Mutation

R594H

Promoter

T7

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

Plasmid

#99229

Purpose

Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos2

Tags

Lifeact

Expression

Mammalian

Mutation

A69T for primed conversion applications

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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