We narrowed to 12,024 results for: pcdna

1. pcDNA3.1V5-His Snk/Plk2 T239D

   Plasmid

   #22220

   Depositor

   Wafik El-Deiry

   Article

   Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

   Insert

   Snk/Plk2 (PLK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   single amino acid substitution mutant, threonine …

   Promoter

   Available Since

   Aug. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry

   Plasmid

   #181851

   Purpose

   mCherry-tagged DnaJB1-PKA catalytic subunit fusion; contains catalytically dead PKA; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   DnaJB1-PKAcat(K72H)-mCherry (PRKACA, DNAJB1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Lysine 128 in the DnaJB1-PKAcat fusion changed to…

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-dCas9-MQ1(S317A)-EGFP

   Plasmid

   #89636

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG and 6*His

   Expression

   Mammalian

   Mutation

   S317A, AGC-->GCC

   Promoter

   CMV

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3580 pcDNA3 Bad S112A S136A

   Plasmid

   #8799

   Depositor

   Stanley Korsmeyer

   Article

   Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

   Insert

   Bad (Bad Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S112A, S136A

   Promoter

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1 Flag-HIF1B [F446L] (#931)

   Plasmid

   #99917

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

   Insert

   HIF1B (ARNT Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F446L

   Promoter

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 SARS-CoV-2 S D614G-trunc

   Plasmid

   #158077

   Purpose

   Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-flag-mKdm4d(H189A)-polyA

   Plasmid

   #61554

   Purpose

   For mRNA synthesis or expression in mammalian cells

   Depositor

   Yi Zhang

   Article

   Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

   Insert

   Kdm4d (catalytic mutant)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   changed Histidine 189 (of Kdm4d) to Alanine

   Promoter

   CMV and T7

   Available Since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 FLAG-SIV-RCM-vpx

   Plasmid

   #115844

   Purpose

   Encodes codon optimized FLAG tagged SIVRCM-Vpx

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SIVRCM-Vpx
   

   Use

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNATM3.1/V5-His TOPO-PKCi-K274W

   Plasmid

   #55690

   Purpose

   expresses dominant negative human PKCi tagged with V5

   Depositor

   Pedro Salas

   Article

   Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

   Insert

   Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   A120E and K274W

   Promoter

   CMV

   Available Since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1-beta-hRLuc-V5-6xbsMS2-MALAT_X

    Plasmid

    #147924

    Purpose

    Mammalian Expression of 6xbsMS2-Malat

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    6xbsMS2-Malat (Malat1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2

    Plasmid

    #212112

    Purpose

    Expression of C-terminally tagged GABARAP(G116A) with mTagBFP2

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP(G116A)-mTagBFP2 (GABARAP Human, Entacmaea quadricolor)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    G116A (lipidation deficiency) of GABARAP

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF

    Plasmid

    #201217

    Purpose

    pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Use

    Tags

    Flag and SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3-lyn10-mCherry- LOVPLD-P (LOVPLD)

    Plasmid

    #219555

    Purpose

    PM-targeted LOVPLD

    Depositor

    Jeremy Baskin

    Article

    Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

    Insert

    lyn10-mCherry-LOVPLD
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-CD81-(TEV)-FKBP-Myc

    Plasmid

    #177908

    Purpose

    human CD81-TEV cleavage site-FKBP-Myc tag

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    human CD81 (CD81 Human, Synthetic)
    

    Use

    Tags

    FKBP, Myc tag, and TEV protease cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3puro-mGFP-ROCK2-C (941-1388)

    Plasmid

    #225471

    Purpose

    Express mEGFP-fusion protein of ROCK2 fragment (941-1388)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    ROCK2 (ROCK2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    C terminal fragment (941-1388)

    Promoter

    CMV

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Flag-RNF168 delta RING

    Plasmid

    #133982

    Purpose

    mammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of RING

    Promoter

    CMV

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA3 Flag MFF iso2 S172A

    Plasmid

    #74392

    Purpose

    Flag tagged human S172A MFF isoform 2 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Promoter

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

18. YFP(1-158)-beta-1 in pcDNAI/Amp

    Plasmid

    #54468

    Purpose

    An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

    Insert

    YFP (1-158)/beta-1 (GNB1 Human, Aequorea victoria)
    

    Use

    Tags

    YFP(1-158) was fused to the amino terminus of Gbe…

    Expression

    Mammalian

    Mutation

    GBeta-1 was amplified via PCR, which removed an i…

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 LIC 6A FLAG-TEV-DPP8

    Plasmid

    #166795

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP8

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP8 (DPP8 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH

    Plasmid

    #22930

    Purpose

    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domain

    Depositor

    Alexandra Newton

    Article

    Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

    Insert

    PHLPP1-deltaPH (PHLPP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes 127-1205 aa. Deleted amino-terminal PH d…

    Promoter

    CMV

    Available Since

    Feb. 3, 2010

    Availability

    Academic Institutions and Nonprofits only