We narrowed to 6,854 results for: itch

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pCDH-CIBN-CAAX

Plasmid

#188989

Purpose

A lentiviral plasmid encoding plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

CIBN (CIB1 Mustard Weed)

Tags

CAAX

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry::LANS1

Plasmid

#60784

Purpose

Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

Depositor

Brian Kuhlman

Article

Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

Insert

LANS1

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Src(YF)-mCherry

Plasmid

#87357

Purpose

PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src isoform 2 (Src Mouse)

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Y535F

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDragon-DTA

Plasmid

#155017

Purpose

For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to attenuated diphtheria toxin (tox176) in the presence of Cre and (r)tTA activity

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Insert

DTA

Use

Mouse Targeting

Mutation

tox176

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-CibN

Plasmid

#60552

Purpose

Expresses dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSR02

Plasmid

#69149

Purpose

Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

rps-27

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR

Plasmid

#32483

Purpose

Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

ChR2HA-2a-PSAML141F,Y115F:GlyR

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

ChR2 2A

Promoter

CAG

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Src(KD)-mCherry

Plasmid

#87358

Purpose

PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src (Src Mouse)

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

D388R

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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bPAC-mycHis_pGEM

Plasmid

#85468

Purpose

Expression of photoactivated cyclase bPAC of Beggiatoa sp.

Depositor

Peter Hegemann

Article

Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

Insert

Bacterial Photoactivated Adenylyl Cyclase

Tags

His and Myc

Promoter

T7 promoter

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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K401-iLid

Plasmid

#122484

Purpose

Component of optically-induced motor dimerization system

Depositor

Matt Thomson

Article

Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

Insert

Kinesin 1-401 iLid His tag

Tags

His6 and iLid

Expression

Bacterial

Mutation

Kinesin amino acids 1-401

Promoter

T7

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

Plasmid

#125225

Purpose

Expresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-NLS

Plasmid

#51571

Purpose

Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

NLS and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-Htb2

Plasmid

#51569

Purpose

Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

HTB2 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-hDcp2

Plasmid

#72216

Purpose

bacterial expression of GST-tagged human Dcp2

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

GST

Expression

Bacterial

Mutation

F146Y and E198K (please see depositor's comm…

Promoter

tac

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pInsert-PhoCl-RER

Plasmid

#219676

Purpose

Caging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2

Depositor

Helge Ewers

Article

Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

Insert

CD4mScarlet-PhoCl-RER

Tags

mScarlet and PhoCl

Expression

Mammalian

Promoter

TRE

Available Since

June 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLX208-gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4

Plasmid

#125230

Purpose

For generating SPARK NanoLuc-oβ2AR transmembrane component lentivirus

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4

Use

Lentiviral

Tags

HA,Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-hDcp2

Plasmid

#72213

Purpose

mammalian expression of Flag-tagged human Dcp2

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Q170R (please see depositor's comment below)

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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S12.1_Empty_Bridge

Plasmid

#127670

Purpose

Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDN43

Plasmid

#61346

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS11 and PKIt NES

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CRY2-mCherry

Plasmid

#178525

Purpose

Expresses CRY2-mCherry in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

CRY2-mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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