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pDisplay FLIPE-10u

Plasmid

#13546

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207R

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207R in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pDisplay FLIPE-1m

Plasmid

#13548

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pLLX13

Plasmid

#79825

Purpose

Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Yeast and bacteria shuttle vector, contains origi…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Prohibitin-bio-His

Plasmid

#50800

Purpose

Expresses enzymatically monobiotinylated full length Prohibitin

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

Prohibitin

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pVIM-BC2T

Plasmid

#108967

Purpose

expresses vimentin-BC2-tag in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

Vimentin

Tags

BC2T

Expression

Mammalian

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTetO-Kir2.1-IRES-TLZ

Plasmid

#32642

Depositor

Richard Axel, Joseph Gogos, C. Ron Yu

Article

Yu et al Neuron. 2004 May 27;42(4):553-66.

Insert

tetO-Kir2.1-IRES-TLZ (Kcnj2 Mouse)

Expression

Bacterial

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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DAP (DOL-AP) (CC#44)

Plasmid

#15164

Depositor

Connie Cepko

Article

Fields-Berry et al Proc Natl Acad Sci U S A. 1992 Jan 15. 8

Insert

PLAP (ALPP Human)

Use

Retroviral; Avian expression

Expression

Mammalian

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
pTS104 pHAGE2 CMVtetO2 MCS-3C-22xGS-GFP

Plasmid

#199357

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-21-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85857

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-21-3p Sensor (MIR21 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pR540

Plasmid

#207885

Purpose

template for 2 gRNA amplification

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

scaffold-pU6

Expression

Bacterial

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCS021

Plasmid

#207917

Purpose

Level 0 for gRNA in E2 module (5' CCAT-3' ACTA)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pKE013

Plasmid

#207916

Purpose

Level 0 for gRNA in E1 module (5' CTGC-3' CCAT)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Salsa-BC82-P1G10

Plasmid

#202923

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC82

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC82

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mTFP1-VSVG-16

Plasmid

#55517

Purpose

Localization: Golgi/ER Transport, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

VSVG

Tags

mTFP1

Expression

Mammalian

Mutation

deltaK VSVG ts045

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
Salsa-BC2-P1A2

Plasmid

#202843

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC2

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC2

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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HOXB6-pCMV

Plasmid

#21258

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

HOXB6 (HOXB6 Human)

Tags

FLAG_Pro Tag

Expression

Bacterial and Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pReporter_13_switchBP

Plasmid

#62848

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). attP upstream of attB in this plasmid.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_13_switchBP

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pUHD Cdk2 WT HA

Plasmid

#27650

Depositor

Greg Enders

Article

Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

Insert

Cdk2 (CDK2 Human)

Tags

HA (YDVPDYA)

Expression

Mammalian

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only
Salsa-BC6-P1A6

Plasmid

#202847

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC6

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC6

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MKK4

Plasmid

#29579

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

MKK4 (MAP2K4 Human)

Tags

GST, HIS6, and TEV

Expression

Bacterial

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only