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pQE30-His-T7RNAP

Plasmid

#124138

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

T7 RNA polymerase (T7p07 Enterobacteria phage T7)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGBScomp High

Plasmid

#223202

Purpose

Group B Streptococcus High Expression Modular Complementation Vector

Depositor

Thomas Hooven

Article

Hillebrand et al Mol Microbiol. 2025 Nov;124(5):449-461.

Insert

N/A

Use

Complementation vector

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Autobow

Plasmid

#45182

Depositor

Joshua Sanes

Article

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pCAG-Autobow

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-Autobow

Plasmid

#45182

Depositor

Joshua Sanes

Article

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pCAG-Autobow

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pSBbi-GP

Plasmid

#60511

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSBbi-GP

Plasmid

#60511

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-HyPer7-MEM

Plasmid

#136465

Purpose

Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Farnselation tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-HyPer7-MEM

Plasmid

#136465

Purpose

Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Farnselation tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro shKRAS

Plasmid

#116871

Purpose

Tet-inducible suppression of KRAS

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

shKRAS (KRAS Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1/TO

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro shKRAS

Plasmid

#116871

Purpose

Tet-inducible suppression of KRAS

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

shKRAS (KRAS Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1/TO

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27

Plasmid

#163617

Purpose

mouse SNX27 cDNA N-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27 (Snx27 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a mNeonGreen-3K-B1-10-IRES-mKate2-2A-PuroR

Plasmid

#215377

Purpose

Lentiviral expression of a cytosolic-localized split mNeonGreen variant that enables efficient complementation when the missing beta strand is present. mKate2 is present as a transduction control.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

mNeonGreen-3K-1-10

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMOS008E: Entry vector for: GCaMP6F calcium sensor (cytosolic)

Plasmid

#163064

Purpose

Entry vector for: GCaMP6F calcium sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

GCaMP6F

Use

Gateway Cloning

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-Opto-zFGFR1a

Plasmid

#232639

Purpose

Zebrafish FGFR1a receptor kinase domain fused to VfLOV

Depositor

Katherine Rogers

Article

Iannucci et al bioRxiv [Preprint]. 2025 Apr 19:2025.04.

Insert

zFGFR1a kinase domain + VfLOV domain

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX neo PFKP-GFP

Plasmid

#116940

Purpose

Expression of PFKP-GFP fusion protein

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

PFKP (PFKP Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX neo PFKP-GFP

Plasmid

#116940

Purpose

Expression of PFKP-GFP fusion protein

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

PFKP (PFKP Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCT5-bac2.0

Plasmid

#119872

Purpose

Cumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

Depositor

Claudia Schmidt-dannert

Article

Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

Inserts

CymR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pveg promoter with CuO sequence and PxylR promoter

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCT5-bac2.0

Plasmid

#119872

Purpose

Cumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

Depositor

Claudia Schmidt-dannert

Article

Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

Inserts

CymR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pveg promoter with CuO sequence and PxylR promoter

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGSHU

Plasmid

#72244

Purpose

Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
hyg
I-SceI

Expression

Bacterial

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pGSHU

Plasmid

#72244

Purpose

Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
hyg
I-SceI

Expression

Bacterial

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pST-K

Plasmid

#44560

Purpose

for ectopic constitutive expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Promoter

UV15 promoter

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pST-K

Plasmid

#44560

Purpose

for ectopic constitutive expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Promoter

UV15 promoter

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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TFORF3325

Plasmid

#144801

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

DLX3 (DLX3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3325

Plasmid

#144801

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

DLX3 (DLX3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-sCD200

Plasmid

#164424

Purpose

AAV plasmid expressing soluble CD200 in retinal pigment epithelium

Depositor

Connie Cepko

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

Insert

CD200 (Cd200 Mouse)

Use

AAV

Expression

Mammalian

Mutation

deleted terminal 40 amino acids

Promoter

Best1

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

Plasmid

#196196

Purpose

homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)

Use

Homology repair donor

Tags

V5-APEX2-mScarlet-I

Available Since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-spCas9-N57

Plasmid

#169920

Purpose

Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.

Depositor

Zhili Rong

Article

Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

Insert

SpCas9-N57

Use

CRISPR

Mutation

N57 is the PAI domain of SB100X

Promoter

CAG

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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T7RNAP Cyslight

Plasmid

#205077

Purpose

T7RNAP protein expression

Depositor

Smita Patel

Article

Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

Insert

T7RNAP Cyslight (T7p07 Bacteriophage T7)

Expression

Bacterial

Mutation

C125S, C347S, C510S, C515S, C530S, C723S

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-p21-HA

Plasmid

#215110

Purpose

Expresses p21-HA in mammalian cells from an inducible lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV (Tet-inducible)

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-PSD95-mCherry

Plasmid

#125694

Purpose

Exogenous expression of PSD95 (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95-mCherry

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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