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pET-DEUT RUVBL1 Delete

Plasmid

#159144

Purpose

Expression and protien purification of mutated RUVBL1

Depositor

Eric Erquan Zhang

Article

Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

Insert

RUVBL1 (RUVBL1 Human)

Expression

Bacterial

Mutation

amino acid 1-463, Glu134-Glu237 mutated to GPPG

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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mVenus-proα2(I)

Plasmid

#119838

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with mVenus between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

mVenus

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag

Promoter

CMV

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A774L

Plasmid

#174796

Purpose

Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A774L (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A774L, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-W318R

Plasmid

#174793

Purpose

Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-W318R (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

W318, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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EFNB2-tdTomato

Plasmid

#139967

Purpose

Donor vector to knockin tdTomado into EFNB2 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

Human EFNB2 knockin homology arms (EFNB2 Human)

Expression

Mammalian

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD27h_2ntmut

Plasmid

#73067

Purpose

expression clone for human mutated SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (E2F7 Human)

Tags

no tag

Expression

Mammalian

Mutation

2 nt mutation in AS box that binds E2F7 pre-mRNA

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-A762V

Plasmid

#174795

Purpose

Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A762V (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A762V

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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tdEos-Actin-C-18

Plasmid

#57571

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Actin (ACTB Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1_hE2F7mg_PP7-tag

Plasmid

#73078

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with PP7-tag on 5'-end

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Tags

5'-PP7-tag RNA

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR1A-PIK3CA E545K

Plasmid

#180032

Purpose

Entry vector of PIK3CA E545K for gateway cloning.

Depositor

Xin Chen

Article

Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

Insert

PIK3CA E545K (PIK3CA Human)

Use

Entry vector

Tags

HA tag

Expression

Mammalian

Mutation

E545K

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC1534 pscAAV mU6 shRNA(PKCd) CMV-IE Nuc-EYFP

Plasmid

#135562

Purpose

An AAV vector expressing shRNA vs rat PKCd and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

shRNA (mouse PKCd)
Nuc-EYFP

Use

AAV and RNAi

Tags

3xNLS

Expression

Mammalian

Promoter

CMV-IE and mU6

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAP1892-1

Plasmid

#105247

Purpose

human GFP11 with tagRFP 33bp downstream in Lamin A/C

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

Insertion of GFP11 at cut tagRFP 33bp downstream in Lamin A/C (LMNA Human)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAP1716-1

Plasmid

#105245

Purpose

human eGFP with homology arms for Lamin A/C

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with homology arms for Lamin A/C (LMNA Human)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBOB-N-3HA-GK_v1

Plasmid

#134275

Purpose

Lentivector encoding 3XHA-tagged GK (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

524 amino acids, isoform 1

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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mCerulean-proα2(I)

Plasmid

#119836

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

mCerulean

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF1CACTA1_3xFLAG_TET1cd

Plasmid

#106434

Purpose

pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

TET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)

Use

Synthetic Biology

Tags

3xFLAG

Expression

Plant

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIK-Neo-TGmiR-Gb2

Plasmid

#25746

Purpose

Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 (Gnb2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)

Plasmid

#114100

Purpose

fusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

Gaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCR-HA-IKKalphad17

Plasmid

#19738

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase alpha (Chuk Mouse)

Tags

HA

Expression

Mammalian

Mutation

Splice variant lacking 17 amino acids at the C-te…

Available Since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only
pET21a-MKP7_K55R_153

Plasmid

#37794

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

Insert

Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)

Tags

His

Expression

Bacterial

Mutation

changed Lysnie 55 to Arginine and deleted amino a…

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only