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pET32a-HD25Q

Plasmid

#11508

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 25 glutamines (HTT Human)

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 25 glutamines, vector m…

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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pTarget

Plasmid

#214052

Purpose

Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

Target sequence

Use

Target rna plasmid

Expression

Bacterial

Promoter

trc promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pHochTypeIII

Plasmid

#214039

Purpose

Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

Cas10

Use

CRISPR

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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MigR1-AE

Plasmid

#12431

Depositor

Dong-Er Zhang

Article

Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

Insert

AML1-ETO fusion (RUNX1, RUNX1T1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

June 2, 2010

Availability

Academic Institutions and Nonprofits only

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pmCherryC2-Gephyrin P1

Plasmid

#68820

Purpose

mCherry tagged Geph for mammalian expression

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al Proc Natl Acad Sci U S A. 2011 Jan 4;108

Insert

Gephyrin (Gphn Rat)

Tags

mCherry

Expression

Mammalian

Mutation

silent mutations on internal EcoRI site. Gephyrin…

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pAG87 FAST

Plasmid

#130719

Purpose

Expresses His-tagged FAST (also called YFAST) in bacteria

Depositor

Arnaud Gautier

Article

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

FAST

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLM-vexGFP-Oct4

Plasmid

#22240

Purpose

2nd generation lentiviral vector

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

vexGFP_2A_OCT4 (POU5F1 Human, synthetic fluorescent protein)

Use

Lentiviral

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pXL002-ishRNA-beta-catenin-1

Plasmid

#36297

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of beta-catenin-1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

IRES-RFP-Puro

Expression

Mammalian

Promoter

TetO-H1

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
SunTag-FWAgRNA-4-22aa-TET1cd

Plasmid

#106435

Purpose

SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR

Plasmid

#135500

Purpose

Mammalian expression of FLAG-tagged TAPBPR

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

TAPBPR (TAPBPL Human)

Tags

Luminal FLAG epitope tag and Signal peptide from …

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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His6tev CBP HAT (1324-1700)

Plasmid

#99340

Purpose

bacterial expression of His tagged mouse CBP HAT domain (1324-1700)

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP HAT domain (1324-1700) (Crebbp Mouse)

Tags

His6tev

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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aav-tnt-wtmrtfa-gfp

Plasmid

#165037

Purpose

AAV-based delivery of wtMRTFA-GFP into cardiomyocytes in vivo

Depositor

William Pu

Article

Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

Mrtfa (Mrtfa Mouse)

Use

AAV

Tags

GFP

Promoter

cTNT

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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3xFLAG-FKBP-LRRK2

Plasmid

#193584

Purpose

Expresses human full-length LRRK2 with FKBP domain and 3xFLAG situated at the N-terminus

Depositor

Mark Cookson

Article

Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Leucine-rich repeat kinase 2 (LRRK2 Human)

Tags

3xFLAG and FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-ARNTL-shRNA1

Plasmid

#166913

Purpose

Lentivirus for inducible knockdown of human ARNTL(BMAL1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNTL shRNA (BMAL1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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G-GECO1.2-Orai1

Plasmid

#73562

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

G-GECO1.2-Orai1 (ORAI1 Synthetic, Human)

Tags

G-GECO1.2

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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NOTCH3-bio-His

Plasmid

#53343

Purpose

Expresses full-length Neurogenic locus notch homolog protein 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

NOTCH3 (NOTCH3 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pET32a-HD16Q

Plasmid

#11487

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 16 glutamines (HTT Human)

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 16 glutamines, vector m…

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-Zn2

Plasmid

#174805

Purpose

Bacterial expression of isolated PARP1 Zn2 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn2 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Zn3

Plasmid

#174806

Purpose

Bacterial expression of isolated PARP1 Zn3 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn3 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLNCX myr Akt1

Plasmid

#17245

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Akt1 (Akt1 Mouse)

Use

Retroviral

Tags

Myr

Expression

Mammalian

Mutation

Constitutively active

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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