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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,796 results for: LEA

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Showing: 5961 - 5980 of 12796 results

  8. pcDNA3-ERKsubDD-Venus-NES

    Plasmid
    #84630
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted
    Depositor
    Jin Zhang
    Publication
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ERKsubDD-Venus-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    84630-plasmid-map-sequence-id-175152

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  9. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid
    #138208
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Publication
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PKCsub(T/A)-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138208-plasmid-map-sequence-id-307411

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  10. pGBW-m4046839

    Plasmid
    #145646
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145646-plasmid-map-sequence-id-323990

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  11. pGBW-m4046770

    Plasmid
    #145594
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 leader protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145594-plasmid-map-sequence-id-323992

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  12. pGBW-m4046797

    Plasmid
    #145676
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 leader protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 27, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145676-plasmid-map-sequence-id-292488

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  16. TVMV-TEV-HRV-3C Proteases-mCherry

    Plasmid
    #243798
    Purpose
    Encodes for mammalian expression of TVMV, TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
    Depositor
    Cole DeForest
    Publication
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TVMV, TEV and HRV-3C proteases
    Expression
    Mammalian
    Available since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243798-plasmid-map-sequence-id-486451

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  18. CT769

    Plasmid
    #233661
    Purpose
    Split Flp on same plasmid; PhyA-Flp-397-Flp-396-FHY1
    Depositor
    Wilson Wong
    Publication
    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PhyA-Flp-397-Flp-396-FHY1
    Expression
    Mammalian
    Mutation
    None
    Available since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    233661-plasmid-map-sequence-id-463755

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Showing: 5961 - 5980 of 12796 results