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We narrowed to 221 results for: sleeping beauty

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Showing: 61 - 90 of 221 results

  1. pSB-Bleo Gal4 tBFP

    Plasmid
    #139190
    Purpose
    Gal4-inducible tag blue fluorescent protein (tBFP) in pSB-Bleo Gal4 Sleeping Beauty backbone.
    Depositor
    Neal Devaraj
    Insert
    tBFP
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    Gal4 CMV and STAT3
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    139190-plasmid-map-sequence-id-309397

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  2. pT2/shαSMA/GFP4_Seq2/clone1

    Plasmid
    #201402
    Purpose
    Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    alpha-SMA
    Expression
    Mammalian
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    201402-plasmid-map-sequence-id-394260

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  3. pSBBi-Pur LaG17-synNotch-Gal4VP64

    Plasmid
    #158135
    Purpose
    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backbone
    Depositor
    Neal Devaraj
    Insert
    LaG17-synNotch-Gal4VP64
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Available Since
    Oct. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158135-plasmid-map-sequence-id-309380

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  4. pSB-Bleo STAT3

    Plasmid
    #139184
    Purpose
    Empty backbone encoding constitutive bleomycin resistance with an inducible STAT3 promoter to allow for an insert in a Sleeping Beauty backbone.
    Depositor
    Neal Devaraj
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    STAT3
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    139184-plasmid-map-sequence-id-309370

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  5. pSBBi-Pur LaG17-synNotch-TetRVP64

    Plasmid
    #158133
    Purpose
    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backbone
    Depositor
    Neal Devaraj
    Insert
    LaG17-synNotch-TetRVP64
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    158133-plasmid-map-sequence-id-309383

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  7. (829) SB Tet-ON dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195622
    Purpose
    Sleeping Beauty Tet-ON plasmid for induced expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    Tight TRE promoter; Tet-ON (doxycycline)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195622-plasmid-map-sequence-id-385115

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  8. (915) SB EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195623
    Purpose
    Sleeping Beauty EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    EF1a (Mlu1/EcoR1)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195623-plasmid-map-sequence-id-385117

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  9. pTC-ApoE-Tet

    Plasmid
    #85578
    Purpose
    Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liver
    Depositor
    Ursula Ehmer
    Article
    Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg
    Insert
    CreER
    Use
    Cre/Lox
    Expression
    Mammalian
    Promoter
    ApoE.HCR.hAAT
    Available Since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    85578-plasmid-map-sequence-id-169842

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  10. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

    Plasmid
    #133779
    Purpose
    Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    Isocitrate dehydrogenase 1 (IDH1 Human)
    Tags
    Myc and FLAG
    Expression
    Mammalian
    Mutation
    Arginine is mutated to histidine at amino acid po…
    Available Since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133779-plasmid-map-sequence-id-261114

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  14. pAF112

    Plasmid
    #135920
    Purpose
    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Type
    Empty backbone
    Use
    Luciferase; Transposon
    Expression
    Mammalian
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135920-plasmid-map-sequence-id-270078

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  27. pSBbi-TIR1-tTA/Pur

    Plasmid
    #171683
    Purpose
    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)
    Depositor
    Randy Poon
    Article
    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.
    Inserts
    tetracycline-controlled transactivator
    Puromycin
    EF-1a promoter
    Expression
    Mammalian
    Promoter
    EF-1a promoter and RPBSA promoter
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    171683-plasmid-map-sequence-id-338224

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Showing: 61 - 90 of 221 results