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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,845 results for: FAST

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Showing: 41 - 60 of 1845 results

  2. pFastBac1-pGC-A

    Plasmid
    #186626
    Purpose
    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)
    Depositor
    Debra Hansen
    Article
    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1
    Insert
    NPR1 (NPR1 Human)
    Tags
    hemagglutinin (HA) signal peptide + His10-tag + T…
    Expression
    Insect
    Mutation
    deleted amino acids 1-32
    Promoter
    polyhedring
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186626-plasmid-map-sequence-id-366906

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  3. pFastBac HT JS-Munc18c

    Plasmid
    #135555
    Purpose
    Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.
    Depositor
    Jingshi Shen
    Article
    Yu et al Methods Mol Biol. 2019;1860:237-249. doi
    Insert
    Munc18c
    Tags
    6xHis tag and TEV site
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    135555-plasmid-map-sequence-id-272498

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  4. SPLICSFAST-PO-MT-P2A

    Plasmid
    #214899
    Purpose
    Expresses equimolar Peroxisome- and Mitochondria- targeted split-FAST fragments for Short-range PO-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-PO-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214899-plasmid-map-sequence-id-423486

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  6. SPLICSFAST-NU-MT-P2A

    Plasmid
    #214898
    Purpose
    Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-NU-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214898-plasmid-map-sequence-id-423484

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  7. pCMV-iGlu3Fast

    Plasmid
    #226500
    Purpose
    Ultrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate release
    Depositor
    Katalin Torok
    Article
    Tran et al bioRxiv, August 30th, 2024
    Insert
    iGluSnFR3 S72T
    Tags
    IgK, Myc tag, and PDGFR Beta TM domain
    Expression
    Mammalian
    Mutation
    S72T
    Promoter
    CMV
    Available Since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    226500-plasmid-map-sequence-id-448688

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  8. pFastBac1-melHis10TEV

    Plasmid
    #159428
    Purpose
    empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Tags
    melittin signal sequence (removed in vivo by sign…
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    159428-plasmid-map-sequence-id-333931

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  11. pFastBac1_FLAG-BPTF

    Plasmid
    #102242
    Purpose
    Expression of human Flag-BPTF using baculovirus from pFastBac1
    Depositor
    Joe Landry
    Insert
    Flag-BPTF (BPTF Human)
    Tags
    Flag
    Expression
    Insect
    Promoter
    Polyhedron
    Available Since
    Dec. 11, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    102242-plasmid-map-sequence-id-197767

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  13. pEND-164_Cacnes_pFAST

    Plasmid
    #225616
    Purpose
    Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    pFAST
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225616-plasmid-map-sequence-id-451365

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  14. pFastBac_Dual_SNF2L-opt_p48

    Plasmid
    #102243
    Purpose
    Expression of human codon optimized human SNF2L and pRbAp48 from pFastBac dual
    Depositor
    Joe Landry
    Inserts
    SNF2L (SMARCA1 Human)
    pRbAp48 (RBBP4 Human)
    Expression
    Insect
    Mutation
    codon optimized for expression
    Promoter
    Polyhedrin and p10
    Available Since
    Nov. 1, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    102243-plasmid-map-sequence-id-197769

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  15. pFastBac1-Nucleocapsid

    Plasmid
    #179696
    Purpose
    Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells
    Depositor
    Mark Arbing
    Insert
    SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    His6-TEV protease site
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179696-plasmid-map-sequence-id-352199

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  17. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

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Showing: 41 - 60 of 1845 results