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Showing: 41 - 60 of 29859 results

  2. pLentiGuide-BlastR

    Plasmid
    #196970
    Purpose
    Lentiviral acceptor vector for pegRNAs with blasticidin resistance
    Depositor
    Leopold Parts
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    196970-plasmid-map-sequence-id-441210

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  5. lentiGuide-Crimson

    Plasmid
    #70683
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Article
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    S. pyogenes sgRNA cassette
    Crimson Reporter
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-a and hU6
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70683-plasmid-map-sequence-id-217910

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  6. pOD1988-epiDEG

    Plasmid
    #89357
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Insert
    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Expression
    Mutation
    Promoter
    Pdpy-7
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    89357-plasmid-map-sequence-id-177228

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  9. Hygro-iDEST

    Plasmid
    #75339
    Purpose
    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression
    Depositor
    Danwei Huangfu
    Article
    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-
    Insert
    TRE-DEST
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75339-plasmid-map-sequence-id-142552

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  12. Puro-iDEST

    Plasmid
    #75336
    Purpose
    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression
    Depositor
    Danwei Huangfu
    Article
    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-
    Insert
    TRE-DEST
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    75336-plasmid-map-sequence-id-149881

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  15. pETSUMO2_Bacteroidetes-bGSDM

    Plasmid
    #212124
    Purpose
    For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Science. 2022 Jan 14;375(6577):221-225.
    Insert
    Bacteroidetes bGSDM
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212124-plasmid-map-sequence-id-419109

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  18. lentiGuideFB-Puro-A

    Plasmid
    #192506
    Purpose
    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:
    Insert
    hU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192506-plasmid-map-sequence-id-375156

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  19. lentiGuideFB-Puro-B

    Plasmid
    #192507
    Purpose
    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:
    Insert
    bU6-sgRNACR2-CS-BsmBI-EFS-Puro-WPRE
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192507-plasmid-map-sequence-id-375157

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  20. mTurquoise-CIDEB-C-18

    Plasmid
    #55543
    Purpose
    Localization: , Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    CIDEB (CIDEB Human)
    Use
    Tags
    mTurquoise
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 41 - 60 of 29859 results