We narrowed to 576 results for: Pef1

1. pEF1α -NWM-Node 3

   Plasmid

   #154255

   Purpose

   expresses reconstructed New World monkey retroCHMP3 Node 3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

   Insert

   reconstructed Node 3 of New World monkey retroCHMP3
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRRLNeo-pEF1a-p53ash-mCerulean

   Plasmid

   #69579

   Purpose

   Expresses p53 tagged with mCerulean

   Depositor

   Galit Lahav

   Article

   Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

   Insert

   p53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   7 silent mutations in p53 DNA to prevent targetin…

   Promoter

   EF1alpha

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. vYM112_PB-pEF1a-optiPIN2-HA(Hygro)

   Plasmid

   #160045

   Purpose

   For expressing the active auxin pump PIN2

   Depositor

   Michael Elowitz

   Article

   Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

   Insert

   PIN2
   

   Use

   Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   pEF1s

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LLP109 pEF1a-gRNA-GFP

   Plasmid

   #100935

   Purpose

   GFP, puromycin and gRNA scaffold driven by EF1a promoter

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   gRNA scaffold
   

   Use

   Grna scaffold

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRLNeo-pEF1a-p53ashL344A-mCerulean

   Plasmid

   #69580

   Purpose

   Expresses p53-L344A mutant tagged with mCerulean

   Depositor

   Galit Lahav

   Article

   Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

   Insert

   p53-L344A (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   p53 L344A mutation that prevents tetramerization …

   Promoter

   EF1alpha

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1α-squirrel-monkey-retroCHMP3-HA

   Plasmid

   #154174

   Purpose

   expresses squirrel monkey retroCHMP3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   retroCHMP3
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRRLNeo-pEF1a-p53ashL344P-mCerulean

   Plasmid

   #69581

   Purpose

   Expresses p53-L344P mutant tagged with mCerulean

   Depositor

   Galit Lahav

   Article

   Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

   Insert

   p53-L344P (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   p53 L344P mutation that prevents oliogomerization…

   Promoter

   EF1alpha

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF1α-spider-monkey-retroCHMP3-HA

   Plasmid

   #154179

   Purpose

   expresses spider monkey retroCHMP3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   retroCHMP3
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. LLP250 pEF1a-TALE-SunTag

   Plasmid

   #100939

   Purpose

   Empty TALE vector fused to SunTagx10 driven by EF1a promoter, with 3xHA tag, no PURO gene

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   TALE insertion site, SunTag array (10xGCN4)
   

   Use

   TALEN

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a_ TET1 CXXC only

    Plasmid

    #124084

    Purpose

    Expression of CXXC domain only form of TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 - CXXC domain (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF1α-owl-monkey-retroCHMP3-HA

    Plasmid

    #154249

    Purpose

    expresses owl monkey retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF1-puro/hpb1(short)

    Plasmid

    #59973

    Purpose

    integrin b1 short headpiece expression (beta-I, hybrid, PSI domains) expression

    Depositor

    Timothy Springer

    Insert

    human integrin b1 (short) (ITGB1 Human)
    

    Use

    Tags

    Base coiled-coil and His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a promoter

    Available since

    Sept. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. LLP251 pEF1a-UNC5C-4-SunTag

    Plasmid

    #100940

    Purpose

    TALE vector with UNC5C-4 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE target sequence for UNC5C, SunTag array (10xGCN4)
    

    Use

    TALEN

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LLP245 pEF1a-TALE-DNMT3a-Wt

    Plasmid

    #100937

    Purpose

    Empty TALE vector driven by EF1a promoter, no Puro, contains DNMT3a catalytic domain

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE insertion site, DNMT3a catalytic domain (DNMT3A Human, Synthetic)
    

    Use

    TALEN

    Tags

    3xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LLP338 pEF1a-DACH1-1-SunTag

    Plasmid

    #100942

    Purpose

    TALE vector with DACH1-1 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE target sequence for DACH1, SunTag array (10xGCN4)
    

    Use

    TALEN

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF1α-mouse-CHMP3(147)-HA

    Plasmid

    #154177

    Purpose

    expresses mouse CHMP3(147) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3 (Chmp3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal truncation of CHMP3 at amino acid 147

    Promoter

    EF1alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. LLP469 pEF1a-mCherry-EMPTY-gRNA

    Plasmid

    #100958

    Purpose

    mCherry driven by EF1a, empty gRNA scaffold driven by U6

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    gRNA empty backbone with RFP
    

    Use

    CRISPR

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

    Plasmid

    #100941

    Purpose

    Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Human, Synthetic)
    

    Use

    Tags

    1xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC

    Plasmid

    #69583

    Purpose

    Expresses p53 tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    7 silent mutations in p53 DNA to prevent targetin…

    Promoter

    EF1alpha

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEf1a OsTIR ires NEO pA T2BH

    Plasmid

    #127910

    Purpose

    Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    OsTIR
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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