Addgene

We narrowed to 21,750 results for: Sis

Showing: 41 - 60 of 21750 results

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pET-SisTnpB1–ωRNA

Plasmid

#228365

Purpose

Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

Depositor

Tao Liu

Article

Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

Insert

SisTnpB1

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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BAP1-wt (siRNA resistant)

Plasmid

#108439

Purpose

Stable expression of BAP1-wt in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

Insert

BAP1 (BAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

c.1641C>T, c.1644T>A, c.1647T>C, c.1650C…

Promoter

CMV

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pRG705_lexO-AsiSI_LexA-TF_LEU2MX

Plasmid

#154817

Purpose

Inducible expression of AsiSI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AsiSI endonuclease
LexA-ER-B112

Use

Tags

FLAG and NLS

Expression

Yeast

Mutation

Promoter

ACT1 and lexO_4-CYC1_core

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TK

Plasmid

#177868

Purpose

Cloning Backbone for mGreenLantern-based EXSISERS containing FRT-sites flanked PuroR-HSV-TK cassette; clone homology arms via BbsI (or BpiI).

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

mGreenLantern-based EXSISERS

Use

CRISPR, Synthetic Biology, and Unspecified

Tags

OLLAS

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-siResist

Plasmid

#49852

Purpose

Encodes human connexin 43 with wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Contains several wobble mutations to confer resis…

Promoter

CMV

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
pRS414 GPD-Sis1

Plasmid

#18687

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 (SIS1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pDONR223 SCAF8_isoC_CRISPR resistant

Plasmid

#122466

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF8 isoform C CRISPR resistant (SCAF8 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Synonymous mutations conferring resistance to gRN…

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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sg resistant gamma-tubulin

Plasmid

#104433

Purpose

For expressing gamma-tubulin in cells expressing γTUBULIN single guide RNA

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

changed t bp28 to c; g bp30 to a; c bp33 to a; …

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pRS414 GPD-Sis1deltaG/F

Plasmid

#18688

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 deltaG/F

Use

Tags

Expression

Yeast

Mutation

Sis1 with the G/F region deleted

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
MPS1 Candia parapsilosis

Plasmid

#232153

Purpose

MPS1 from Candia parapsilosis codon optimized for S. cerevisiae in pUCIDT

Depositor

Mark Winey

Article

Fabritius et al PLoS One. 2024 Mar 26;19(3):e0301084. do

Insert

MPS1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHIV(T9)-DERA-sgresis

Plasmid

#222622

Purpose

Lentiviral vector that expresses sgRNA resistant DERA in mammalian cells

Depositor

Daniel Durocher

Article

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

DERA (DERA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Lenti-sgSis#2/Cre

Plasmid

#193238

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Sis gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgSis#2 (Sis Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSis#1/Cre

Plasmid

#193237

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Sis gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgSis#1 (Sis Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-rUSP19ER-siRNAresistant

Plasmid

#155251

Purpose

for mammalian overexpression of the siRNA resistant ER isoform of rat USP19

Depositor

Simon Wing

Insert

USP19 (ER isoform) siRNA resistant (Usp19 Rat)

Use

Tags

6XHis-3XFlag

Expression

Mammalian

Mutation

Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …

Promoter

CMV

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-rUSP19CYT-siRNAresistant

Plasmid

#155252

Purpose

for mammalian overexpression of the siRNA resistant CYT isoform of rat USP19

Depositor

Simon Wing

Insert

USP19 (CYT isoform) siRNA resistant (Usp19 Rat)

Use

Tags

6XHis-3XFlag

Expression

Mammalian

Mutation

Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …

Promoter

CMV

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SCAF4_CRISPR resistant

Plasmid

#122465

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF4 CRISPR resistant (SCAF4 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Synonymous mutations conferring resistance to gRN…

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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p35S::SISOBIR1-YFPc

Plasmid

#86164

Purpose

SOBIR1 split YFP

Depositor

Silke Robatzek

Article

Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

Insert

SOBIR1 (SOBIR1 Mustard Weed)

Use

Tags

YFPc

Expression

Plant

Mutation

Promoter

35S

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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p35S::SISOBIR1-YFPn

Plasmid

#86163

Purpose

SOBIR1 split YFP

Depositor

Silke Robatzek

Article

Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

Insert

SOBIR1

Use

Tags

YFPn

Expression

Plant

Mutation

Promoter

35S

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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CpcB-Picea-sitchensisPHLS_CpcA

Plasmid

#74001

Purpose

Expresses the Picea sitchensis PHLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

Insert

PHLS and CmR

Use

Tags

Expression

Mutation

Promoter

cpc

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-GFP-I3(resist)

Plasmid

#61400

Purpose

Expresses GFP-tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

silent mutations of bases C183T T192C T189C

Promoter

CMV

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only