We narrowed to 860 results for: trna

1. pUC19-U6-tRNAx2

   Plasmid

   #123641

   Purpose

   Mammalian expression, Genome editing, backbone

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. ptRNAfMet-A37U

   Plasmid

   #196659

   Purpose

   Plasmid for overexpression of mutated initiator tRNA (A37U)

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

   Insert

   tRNAfMet-A37U expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A37U in tRNAfMet

   Promoter

   metY native promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. ptRNAfMet-A37G

   Plasmid

   #196658

   Purpose

   Plasmid for overexpression of mutated initiator tRNA (A37G)

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

   Insert

   tRNAfMet-A37G expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A37G in tRNAfMet

   Promoter

   metY native promoter

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTR(EF)-tRNA(Q)

   Plasmid

   #170517

   Purpose

   PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Insert

   gRNAcore(EF)-tRNA(Q)
   

   Use

   Pcr template vector for amplifying grnacore(ef)-t…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mm-PylRS-AF/Pyl-tRNACUA

   Plasmid

   #122650

   Purpose

   tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   PylRS-AF, Pyl-tRNACUA
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

   Promoter

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUP-(2xCouRS-tRNAopt)

   Plasmid

   #197930

   Purpose

   Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

   Depositor

   Kenneth Johnson

   Article

   Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

   Inserts

   CouRS
   tRNA opt
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   glnS' and lambda(ts) and proK

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. SepRS(2)/pSertRNA(B4)/EF-Sep

   Plasmid

   #173897

   Purpose

   The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

   Depositor

   Jason W Chin

   Article

   Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

   Inserts

   SepRS(2)
   pSertRNA
   EF-Sep
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG424-10X-tRNA

   Plasmid

   #70125

   Purpose

   Yeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae introns

   Depositor

   Jay Hesselberth

   Article

   Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

   Insert

   10X-tRNA block
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   None – SUP4 pol III promoter provided by insert

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pYPQ142-ZmUbi-tRNA

   Plasmid

   #158578

   Purpose

   Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAs

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Maize ubiquitin 1

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. NM1-5S-tRNA-SgH

    Plasmid

    #128178

    Purpose

    Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

    Depositor

    Huimin Zhao

    Article

    Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

    Insert

    gRNA cloning cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    5S rRNA-tRNA(Arg)

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ145-ZmUbi-tRNA

    Plasmid

    #158403

    Purpose

    Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAs

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYPQ143-ZmUbi-tRNA

    Plasmid

    #158400

    Purpose

    Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ133-tRNA2.0; assembly of 3 gRNAs

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ146-ZmUbi-tRNA

    Plasmid

    #158404

    Purpose

    Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAs

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQ144-ZmUbi-tRNA

    Plasmid

    #158402

    Purpose

    Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAs

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBS-tRNA-Ser

    Plasmid

    #174318

    Purpose

    For in vitro transcription of mouse tRNAs

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    tRNA-ser
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX458-tRNA-SpCas9

    Plasmid

    #195183

    Purpose

    Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.

    Depositor

    Stephanie Roessler

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    tRNA-Gln

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRANS-Pho2-1/2-2-tRNA

    Plasmid

    #161762

    Purpose

    pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)

    Depositor

    Shaun Curtin

    Article

    Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

    Insert

    Cas9 and gRNAs
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CmYLCV Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOD_B-Pho2-1/2-2-tRNA

    Plasmid

    #161761

    Purpose

    MODULE B tRNA vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2303 - #91068)

    Depositor

    Shaun Curtin

    Article

    Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

    Insert

    gRNAs
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CmYLCV Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS-tRNA-Gly

    Plasmid

    #174317

    Purpose

    For in vitro transcription of mouse tRNAs

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    tRNA-gly
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103_KARS_tRNA-anti-codon

    Plasmid

    #109865

    Purpose

    Protein expression and purification of KARS_tRNA-anti-codon

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    KARS_tRNA-anti-codon (KARS1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge