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AAV_Actb HMEJ donor

Plasmid

#97318

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb NHEJ donor

Plasmid

#97316

Purpose

NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb NHEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb MMEJ donor

Plasmid

#97315

Purpose

MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb MMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Cdx2 HMEJ donor

Plasmid

#97319

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Cdx2. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Cdx2 HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Efs_hSpCas9_NLS_FLAG-SV40

Plasmid

#97307

Purpose

AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

humanized S. pyogenes Cas9

Use

AAV and Mouse Targeting

Tags

Flag

Expression

Mammalian

Promoter

EFS

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

Plasmid

#97313

Purpose

Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

humanized S. pyogenes Cas9

Use

Lentiviral and Mouse Targeting

Tags

Flag

Expression

Mammalian

Promoter

EFS

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Nanog HMEJ donor

Plasmid

#97321

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Nanog. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Nanog HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Sox2 HMEJ donor

Plasmid

#97322

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Sox2. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Sox2 HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Dbh HMEJ donor

Plasmid

#97320

Purpose

HMEJ donor for fusing a p2A-mCherry-WPRE reporter to mouse Dbh. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Dbh HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pKSI-1

Plasmid

#51725

Purpose

cloning backbone

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97308

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97311

Purpose

MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb MMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97312

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97309

Purpose

HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HR donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97310

Purpose

NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb NHEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pMDISI

Plasmid

#51653

Purpose

E coli genetic modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

spcR

Use

Genetic modification

Promoter

unknown

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMDICI

Plasmid

#51652

Purpose

E coli genetic modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

CmR

Use

Genetic modification

Promoter

unknown

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only
pMDIAI

Plasmid

#51655

Purpose

E coli genetic modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

apramycin antibiotic resistance gene

Use

Genetic modification

Promoter

unknown

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pREDTAI

Plasmid

#51627

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

trc

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pREDTKI

Plasmid

#51628

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

trc

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pREDIA

Plasmid

#51625

Purpose

E. coli genome modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

pRha

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pREDKI

Plasmid

#51626

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

pRha

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ256

Plasmid

#124312

Purpose

Gateway entry vector with PmCDA1 and pcoCas9D10A for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9D10A-PmCDA1-UGI

Use

CRISPR

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSwith-tdTomato

Plasmid

#60953

Purpose

Cre recombinase-mediated cassette exchange

Depositor

Liangxue Lai

Article

Li et al Cell Res. 2014 Apr;24(4):501-4. doi: 10.

Insert

tdTomato

Use

Cre/Lox

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ255

Plasmid

#124310

Purpose

Gateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

rAPOBEC1-pcoCas9D10A-UGI

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ150-x3.7

Plasmid

#124308

Purpose

Gateway entry vector with pcoCas9-x3.7 (Plant codon optimized)

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9-x3.7

Use

CRISPR

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ151D10A-x3.7

Plasmid

#124309

Purpose

Gateway entry vector with pcoCas9D10A-x3.7 (Plant codon optimized)

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9D10A-x3.7

Use

CRISPR

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pST1374-GCN4-D10A

Plasmid

#113022

Purpose

Expresses GCN4-D10A in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

GCN4-D10A (GCN4 S. pyogenes, Budding Yeast)

Expression

Mammalian

Mutation

D10A

Promoter

CMV

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pST1374-D10A-GCN4

Plasmid

#113025

Purpose

Expresses D10A-GCN4 in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

D10A-GCN4 (GCN4 S. pyogenes, Budding Yeast)

Expression

Mammalian

Mutation

D10A

Promoter

CMV

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ255-x3.7

Plasmid

#124311

Purpose

Gateway entry vector with rAPOBEC1 and pcoCas9D10A-x3.7 for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

rAPOBEC1-pcoCas9D10A_x3.7-UGI

Use

CRISPR

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ256-x3.7

Plasmid

#124313

Purpose

Gateway entry vector with PmCDA1 and pcoCas9D10A-x3.7 for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9D10A_x3.7-PmCDA1-UGI

Use

CRISPR

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
sAJM.1504

Bacterial Strain

#108251

Purpose

Marionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10B

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Bacterial Resistance

Chloramphenicol

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only
sAJM.1506

Bacterial Strain

#108254

Purpose

Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Bacterial Resistance

Chloramphenicol

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only
sAJM.1505

Bacterial Strain

#108253

Purpose

Marionette-Pro. Marionette cluster inserted in the direction of leading strand replication, between 3,720,027 and 3,721,644 in E. coli BL21

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Bacterial Resistance

Chloramphenicol

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only
ML3

Bacterial Strain

#61907

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. hisG gene deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

Kanamycin

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
ML8

Bacterial Strain

#61909

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. argH gene deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

Kanamycin

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pST1374-scFv-APOBEC-UGI-GB1

Plasmid

#113029

Purpose

Expresses scFv-rAPOBEC1-UGI in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

scFv-rAPOBEC1-UGI-GB1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pST1374-GCN4-dCas9

Plasmid

#113023

Purpose

Expresses GCN4-dCas9 in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

GCN4-dCas9 (GCN4 S. pyogenes, Budding Yeast)

Expression

Mammalian

Mutation

D10A, D839A, H840A, N863A

Promoter

CMV

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pST1374-dCas9-GCN4

Plasmid

#113026

Purpose

Expresses dCas9-GCN4 in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

dCas9-GCN4 (GCN4 S. pyogenes, Budding Yeast)

Expression

Mammalian

Mutation

D10A, H840A, N863A

Promoter

CMV

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pST1374-GCN4-H840A

Plasmid

#113024

Purpose

Expresses GCN4-H840A in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

GCN4-H840A (GCN4 S. pyogenes, Budding Yeast)

Expression

Mammalian

Mutation

D839A, H840A, N863A

Promoter

CMV

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(-)-HF2-BE2

Plasmid

#120396

Purpose

Expresses HF2-BE2 in mammalian cells

Depositor

Junjiu Huang

Article

Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

Insert

HF2-BE2

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pST1374-H840A-GCN4

Plasmid

#113027

Purpose

Expresses H840A-GCN4 in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

H840A-GCN4 (GCN4 S. pyogenes, Budding Yeast)

Expression

Mammalian

Mutation

D839A, H840A, N863A

Promoter

CMV

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(-)-HF2-BE3

Plasmid

#120397

Purpose

Expresses HF2-BE3 in mammalian cells

Depositor

Junjiu Huang

Article

Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

Insert

HF2-BE3

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSY14-ptb

Plasmid

#90490

Purpose

pSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in Clostridium

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Insert

sceC

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pXY1-spc-ptb

Plasmid

#90489

Purpose

pXY1-spc derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in Clostridium

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Insert

sceC

Expression

Bacterial

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-puro-CMV-VC3AI

Plasmid

#78907

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

VC3AI

Use

Lentiviral

Tags

HA and Myc

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-puro-CMV-RC3AI

Plasmid

#78908

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

RC3AI

Use

Lentiviral

Tags

HA

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pCDH-puro-CMV-GC3AI

Plasmid

#78910

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

GC3AI

Use

Lentiviral

Tags

HA and Myc

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-puro-CMV-CC3AI

Plasmid

#78909

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

CC3AI

Use

Lentiviral

Tags

HA and Myc

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pNICKclos1.0

Plasmid

#73639

Purpose

Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sGRNA to pyrE

Use

E.coli-clostridium shuttle vector

Mutation

D10A

Promoter

j23119 and ptb

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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