We narrowed to 757 results for: PI

1. pKM-U6-reRNA-mmDNMT1

   Plasmid

   #205635

   Purpose

   reRNA guide targeting Mus Musculus DNMT1

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   reRNA-mmDNMT1 (Dnmt1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCdc45-mCherry

   Plasmid

   #211380

   Purpose

   mammalian expression of human Cdc45 C-terminally fused to mCherry

   Depositor

   Helmut Pospiech

   Insert

   Cdc45 (CDC45 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19_CDC45_dTAG

   Plasmid

   #232733

   Purpose

   Generates CDC45 dTAG C ternimal knock-in cell lines as donor

   Depositor

   Junjie Chen

   Insert

   CDC45 (CDC45 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Synonymous mutation

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. ADH4

   Plasmid

   #38910

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ADH4 (ADH4 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-Cdc45-mVen

   Plasmid

   #211390

   Purpose

   mammalian expression of human Cdc45 C-terminally fused to mVenus

   Depositor

   Helmut Pospiech

   Insert

   Cdc45 (CDC45 Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Cdc45-mCer

   Plasmid

   #211389

   Purpose

   mammalian expression of human Cdc45 C-terminally fused to mCerulean

   Depositor

   Helmut Pospiech

   Insert

   Cdc45 (CDC45 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-dCas9-p300 Core

   Plasmid

   #61357

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-dCas9-p300 Core (D1399Y)

   Plasmid

   #61358

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. p_sc-eVIP

   Plasmid

   #168174

   Purpose

   Expresses barcoded coding genetic variants in mammalian cells

   Depositor

   James T. Neal, Aviv Regev, Jesse Boehm

   Article

   Ursu et al Nat Biotechnol. 2022 Jun;40(6):896-905.

   Insert

   TP53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Wildtype

   Promoter

   EF-1a

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-dCas9-p300 Core (Y1467F)

    Plasmid

    #61362

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid

    #61361

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459_CDC45_sgRNA

    Plasmid

    #232732

    Purpose

    Generates CDC45 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    CDC45 (CDC45 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMuc1

    Plasmid

    #73929

    Purpose

    Muconate expression plasmid. Expresses enzymes enabling production of muconate from glucose.

    Depositor

    George Church

    Article

    Rogers et al Proc Natl Acad Sci U S A. 2016 Feb 8. pi

    Insert

    muconate producing enzymes
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid

    #61364

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald

    Plasmid

    #203798

    Purpose

    Lentiviral vector expressing human PIK3CA H1047R under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Article

    Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d

    Inserts

    PIK3CA (PIK3CA Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid

    #61359

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid

    #61363

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCB6-BHAPI

    Plasmid

    #99350

    Purpose

    BHA-PI (gpi anchored X:31 HA)

    Depositor

    Judith White

    Article

    Armstrong et al J Cell Biol. 2000 Oct 16;151(2):425-37.

    Insert

    gpi anchored X:31 HA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJKR-H-benM

    Plasmid

    #73925

    Purpose

    Muconate biosensor. Expresses GFP proportional to muconate concentration within the cell.

    Depositor

    George Church

    Article

    Rogers et al Proc Natl Acad Sci U S A. 2016 Feb 8. pi

    Insert

    muconate biosensing apparatus
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK11-01

    Plasmid

    #99605

    Purpose

    Plasmid containing parBp-mKate2 fusion

    Depositor

    Morten Kjos, Jan-Willem Veening

    Article

    van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

    Insert

    parBp-mKate2
    

    Use

    Synthetic Biology

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    PczcD

    Available since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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