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pAAV-Syn-NES-SomaKGECO1

Plasmid

#232839

Purpose

AAV transfer plasmid for Syn-mediated expression of soma-targeted KGECO1

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-SomaKGECO1

Use

AAV

Tags

nuclear export sequence

Expression

Mammalian

Promoter

Syn

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-6xHis-TEV-NDP52

Plasmid

#190194

Purpose

Plasmid for the protein expression of 6xHis-TEV-NDP52 in a bacterial expression system. Internal reference: SMC401

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

NDP52 (CALCOCO2 Human)

Tags

6xHis-TEV

Expression

Bacterial

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRFP2-P2A-HO1-N1

Plasmid

#178966

Purpose

Expresses miRFP2 and HO1 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2-P2A-HO1

Tags

no

Expression

Mammalian

Mutation

NO

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S111F-msfGFP

Plasmid

#180340

Purpose

mammalian expression of human SEPT9_i1 S111F fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S111F

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 R106W-msfGFP

Plasmid

#180339

Purpose

mammalian expression of human SEPT9_i1 R106W fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 R106W

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 D10-25-msfGFP

Plasmid

#180334

Purpose

mammalian expression of human SEPT9_i1 D10-25 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 D10-25

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 DN7-msfGFP

Plasmid

#180335

Purpose

mammalian expression of human SEPT9_i1 DN7 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 DN7

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 NCmut2-msfGFP

Plasmid

#180330

Purpose

mammalian expression of human SEPT9_i1 NCmut2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 I281D, M288D

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-2B-Nkx2-5YRD Y-A

Plasmid

#98611

Purpose

Gateway entry vector for NKX2-5 YRD Y-A

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 YRD Y-A (Nkx2-5 Mouse)

Use

Entry vector

Mutation

Substitutions of 7 tyr into Ala residues.Y233A; Y…

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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EF.STAT3C.Ubc.GFP

Plasmid

#24983

Purpose

3rd generation lentiviral expression of constitutively active Stat3

Depositor

Linzhao Cheng

Article

Hillion et al Cancer Research 68, 10121, December 2008

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

A662C, N664C (constitutively active)

Available Since

May 28, 2010

Availability

Academic Institutions and Nonprofits only

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5` CC: Puro – loxP – GFP-C

Plasmid

#219563

Purpose

5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

Depositor

Andrea Ventura

Article

Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

Insert

hPGK-promoter_PuroR_2A_loxP_GFP-C

Use

Unspecified

Promoter

hPGK

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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3` CC: Hygro – GFP-N – loxP – mScarlet

Plasmid

#219564

Purpose

3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome

Depositor

Andrea Ventura

Article

Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

Insert

3` CC: Hygro – GFP-N – loxP – mScarlet

Use

Unspecified

Promoter

EF1-a

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NES-FRCaMPi-P2A-mGreenLantern

Plasmid

#232841

Purpose

AAV transfer plasmid for CAG-mediated co-expression of FRCaMPi and mGreenLantern

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-FRCaMPi

Use

AAV

Tags

nuclear export sequence

Expression

Mammalian

Promoter

CAG

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pEXP5-aHL_spytag_his

Plasmid

#107334

Purpose

Monomeric SpyTag-6×-His alpha-hemolysin

Depositor

George Church

Article

Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

alpha-hemolysin-SpyTag-6xHis

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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p1303 DV-SFTPC-tdTomato

Plasmid

#126696

Purpose

donor vector for targeting a tdTomato reporter to the human SFTPC locus at the endogenous ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

tdTomato

Expression

Mammalian

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-NES-SomaFRCaMPi

Plasmid

#232837

Purpose

AAV transfer plasmid for Syn-mediated expression of soma-targeted FRCaMPi

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Has Service

AAV8

Insert

NES-SomaFRCaMPi

Use

AAV

Tags

nuclear export sequence

Expression

Mammalian

Promoter

Syn

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMP71-HLA-B0702-His

Plasmid

#104850

Purpose

HLA-B0702 his tagged

Depositor

Sébastien Walchli

Article

Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

Insert

major histocompatibility complex, class I, B (HLA-B Human)

Use

Retroviral

Tags

his

Expression

Mammalian

Promoter

LTR

Available Since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-NES-SomaFRCaMPi

Plasmid

#232836

Purpose

AAV transfer plasmid for CAG-mediated Cre-dependent expression of soma-targeted FRCaMPi

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-FRCaMPi

Use

AAV

Tags

nuclear export sequence

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-NES-SomajRGECO1a

Plasmid

#232842

Purpose

AAV transfer plasmid for Syn-mediated expression of soma-targeted jRGECO1a

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-SomajRGECO1a

Use

AAV

Tags

nuclear export sequence, 6xHis, T7, Xpress tag

Expression

Mammalian

Promoter

Syn

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 R10A R15A-msfGFP

Plasmid

#180336

Purpose

mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 R10A R15A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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