We narrowed to 27,410 results for: sta

1. His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

   Plasmid

   #222991

   Purpose

   Bacterial expression of iGeoCas9(C2) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
   

   Use

   CRISPR

   Tags

   CL7, His, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUASTattB-dALFANb(3mut)-mGreenLantern

   Plasmid

   #219662

   Purpose

   Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3puro-mGFP-FXR1a-sh5Resistant

   Plasmid

   #225448

   Purpose

   Express mEGFP-fusion protein of FXR1 isoform a with shRNA5-resistant silent mutation

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 isoform a (FXR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pST39-Hrs-STAM1 core

   Plasmid

   #21497

   Depositor

   James Hurley

   Article

   Ren et al Structure. 2009 Mar 11. 17(3):406-16.

   Insert

   pST39- Hrs(398-514)-His/STAM1(301-387) (STAM, HGS Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Feb. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

5. qTAG-C-Solo-mStayGold-TOMM20

   Plasmid

   #227307

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. BAP1-C91A (siRNA resistant)

   Plasmid

   #108438

   Purpose

   Stable expression of BAP1-C91A (catalytically inactive mutant) in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

   Insert

   BAP1 (BAP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C91A: c.271T>G, c.272G>C; others: c.1641C&g…

   Promoter

   CMV

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-RFP-p2a-postASAP

   Plasmid

   #178793

   Purpose

   Expresses voltage sensor targeted to dendrites and spines along with RFP

   Depositor

   Rafael Yuste

   Article

   Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

   Insert

   FingR.PSD95, ASAP2f and TagRFP
   

   Use

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   mutations in amino acids L146G, S147T N149R, S150…

   Promoter

   CAG

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Tsapphire-StagRFP-SrcAR

   Plasmid

   #173018

   Purpose

   Long-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   Tsapphire-StagRFP-SrcAR
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLYS1-EMRE/RNAi resistant

   Plasmid

   #50056

   Purpose

   Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi

   Depositor

   Vamsi Mootha

   Article

   Sancak et al Science. 2013 Nov 14.

   Insert

   EMRE-RNAi resistant (SMDT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RNAi resistant, silent mutant

   Promoter

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-5xUASG-iRFP-B3-timestamp-A

    Plasmid

    #159925

    Purpose

    timestamp Template A inserted into the 3’ UTR of iRFP, in a second generation lentiviral backbone with the Vivid promoter, with RNA barcode CTG. AKA 149B3.

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    iRFP-Timestamp A
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Venus2-Y705F/S727A-STAT3

    Plasmid

    #123177

    Purpose

    Expresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    

    Use

    Tags

    Venus2 (aa 158-238) for bimolecular fluorescence …

    Expression

    Mammalian

    Mutation

    Double Y705F and S727A substitutions

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK284 (Stag-3FLAG-Hygro)

    Plasmid

    #72800

    Purpose

    C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    Stag-3FLAG-Hygro
    

    Use

    CRISPR

    Tags

    Stag-3FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR FL CPAP RNAi resistant

    Plasmid

    #46390

    Depositor

    Pierre Gonczy

    Article

    Kitagawa et al J Cell Sci. 2011 Nov 15;124(Pt 22):3884-

    Insert

    CPAP RNAi resistant mutant (CENPJ Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    silent mutation that renders it resistant to siRN…

    Promoter

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

14. qTAG-N-Solo-mStayGold-MYO1C

    Plasmid

    #227306

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-N-Puro-sTagRFP-TUBA1B

    Plasmid

    #207769

    Purpose

    Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pMK283 (Stag-3FLAG-Neo)

    Plasmid

    #72799

    Purpose

    C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    Stag-3FLAG-Neo
    

    Use

    CRISPR

    Tags

    Stag-3FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-N-Puro-mStayGold-ACTB

    Plasmid

    #227320

    Purpose

    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-C-Solo-mStayGold-CLTC

    Plasmid

    #227313

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CLTC Homology Arms flanking a mStayGold Tag (CLTC Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-C-Solo-mStayGold-PXN

    Plasmid

    #227316

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    PXN Homology Arms flanking a mStayGold Tag (PXN Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-VPR-dSpCas9[StaPL(TI)]

    Plasmid

    #111508

    Purpose

    Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].

    Depositor

    Michael Lin

    Article

    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

    Insert

    VPR-dSpCas9[StaPL(TI)]
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The HCV NS3 protease carries F43L, Q80K, and S122…

    Promoter

    PGK

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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