166,461 results

1. pAXY0013

   Plasmid

   #205582

   Purpose

   NpuDnaE(C)_HYG(53-341)_RUBY

   Depositor

   Xiaohan Yang

   Article

   Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

   Insert

   NpuDnaE(C)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAR014 Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD

   Plasmid

   #195548

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. GW1-pHRed

   Plasmid

   #31473

   Purpose

   genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

   Depositor

   Gary Yellen

   Article

   Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

   Insert

   pHRed
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti CMV rtTA3 Hygro (w785-1)

   Plasmid

   #26730

   Purpose

   3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 

   Depositor

   Eric Campeau

   Insert

   Tetracycline repressor A3 mutant
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. Venus1-WTSTAT3

   Plasmid

   #123164

   Purpose

   Expresses wild type STAT3 fused to the 1-157 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
   

   Use

   Tags

   Venus1 (aa 1-158) for bimolecular fluorescence co…

   Expression

   Mammalian

   Mutation

   none

   Promoter

   pCMV

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. SIRT3 Flag

   Plasmid

   #13814

   Purpose

   Mammalian expression of human SIRT3 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT3 (SIRT3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-LbNOX

   Plasmid

   #75285

   Purpose

   This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

   Insert

   LbNOX
   

   Use

   Tags

   C-terminal FLAG tag with linker

   Expression

   Mutation

   Human codon optimized

   Promoter

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (AAV9)

   Viral Prep

   #174007-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (#174007). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s and soma-targeted ChrimsonR. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Mark Histed

   Article

   LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

   Promoter

   Syn

   Tags

   None

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

9. pcVpx.myc

   Plasmid

   #213388

   Purpose

   Generate lentiviral vector which increases gene delivery into primary human myeloid cells

   Depositor

   Nathaniel Landau

   Article

   Sunseri et al J Virol. 2011 Jul;85(13):6263-74. doi: 1

   Insert

   SIVmac vpx (vpx Simian Immunodeficiency Virus)
   

   Use

   Lentiviral

   Tags

   myc-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid

    #85165

    Purpose

    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.

    Depositor

    Robin Dowell

    Article

    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

    Insert

    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    

    Use

    Tags

    AmilCP and RTX

    Expression

    Bacterial

    Mutation

    Promoter

    J23100 promoter

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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