We narrowed to 16,624 results for: puromycin

1. pBabe puro RalA S31N

   Plasmid

   #19718

   Depositor

   Channing Der

   Article

   Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

   Insert

   V-ral simian leukemia viral oncogene homolog A (RALA Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   S31N mutant for dominant neg function. NOTE: lo…

   Available Since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

2. TRAF6mut-UTR

   Plasmid

   #15094

   Depositor

   David Baltimore

   Article

   Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

   Insert

   TRAF6 3'UTR (TRAF6 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   4-bp substitutions in the miR-146 target sites

   Available Since

   June 6, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pMXspuro-GFP-NBR1-dUBA

   Plasmid

   #74205

   Purpose

   Retroviral expression of GFP-NBR1 with aa 877 mutation to generate a premature stop codon

   Depositor

   Jayanta Debnath

   Article

   Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

   Insert

   neighbor of BRCA1 gene 1 (NBR1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed aa 877 to encode for premature stop codon

   Available Since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

4. p6868 MSCV-IP N-V5 Zer1

   Plasmid

   #35100

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   Zer1 (ZER1 Human)
   

   Use

   Retroviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   March 9, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pLV_PP1-mCitrine

   Plasmid

   #172472

   Purpose

   Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

   Depositor

   Sean R Collins

   Article

   Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

   Insert

   prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
   

   Use

   Lentiviral

   Tags

   mCitrine and prolactin signal sequence (cleaved p…

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p6644 MSCV-P C-Flag HA 45E7-Kozak

   Plasmid

   #35135

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   HPV45 E7
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   March 9, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pBabe puro H-Ras G12V Y40C

   Plasmid

   #12276

   Depositor

   Bob Weinberg

   Article

   Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

   Insert

   H-Ras G12V Y40C (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Contains G12V activating mutation. The Y40C mutat…

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

   Plasmid

   #99144

   Purpose

   Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

   Depositor

   Robert Bradley

   Article

   Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   First 127 codons are optimized to reduce GC conte…

   Promoter

   pTight

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Orai1

   Plasmid

   #12199

   Depositor

   Anjana Rao

   Article

   Feske et al Nature. 2006 May 11. 441(7090):179-85.

   Insert

   Orai1 (ORAI1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

10. LentiCRISPR-sgUBA5

    Plasmid

    #86132

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting UBA5

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    sgRNA targeting UBA5 (UBA5 )
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSMP-G9A_4

    Plasmid

    #36334

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    G9A (EHMT2 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pBABE-puro-mIR-31 sponge

    Plasmid

    #22694

    Purpose

    Stable suppression of miR-31 function

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    miR-31 (MIR31 Human)
    

    Use

    Retroviral

    Tags

    *

    Expression

    Mammalian

    Mutation

    miR-31 sponge with 7x tandem binding sites to the…

    Available Since

    March 11, 2010

    Availability

    Academic Institutions and Nonprofits only

13. 3xFlag-HaloTag-EZH2 HDR - pDY053

    Plasmid

    #171108

    Purpose

    Homology directed repair plasmid for generating HaloTag-EZH2 cells

    Depositor

    Thomas Cech

    Article

    Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

    Insert

    EZH2 (EZH2 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe-Sik-WT

    Plasmid

    #210722

    Purpose

    Construct for stable expression of mouse wild type Ptk6 (Sik) in mammalian cells.

    Depositor

    Angela Tyner

    Article

    Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

    Insert

    Protein tyrosine kinase 6 (Ptk6 Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-EF1a-V5-Smpd4

    Plasmid

    #120237

    Purpose

    Expresses V5-tagged Smpd4 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Smpd4 (Smpd4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_4xMma PylT(UUA)_FLAG-AcKRS

    Plasmid

    #140010

    Purpose

    Plasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    AcKRS-3 (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L301M, L305I, Y306F, L309A, C348F

    Promoter

    EF1

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO-puro human lipin 1-1 shRNA

    Plasmid

    #32019

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pINT-PuroDDHNF4a2

    Plasmid

    #31443

    Depositor

    Gerhart Ryffel

    Article

    Waldner et al BMC Res Notes. 2011 Oct 18;4:420.

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Phic31/attb

    Tags

    FKBP L106P

    Expression

    Mammalian

    Available Since

    Jan. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-Puro-TDP-43-D169G

    Plasmid

    #133757

    Purpose

    Lentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed aspartate 169 to glycine.

    Promoter

    CMV

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

    Plasmid

    #40571

    Depositor

    Daniel Gerlich

    Article

    Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

    Insert

    Aurora B FRET-sensor N-terminally fused to histone 2B
    

    Tags

    CyPet and YPet

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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