We narrowed to 10,107 results for: transfer

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AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97309

Purpose

HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HR donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hsChRmine-eYFP-WPRE

Plasmid

#183530

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

hsChRmine-eYFP

Use

AAV

Mutation

H33R

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Tbg-CES2A-ΔC-S227A

Plasmid

#200560

Purpose

Secreted mouse CES2A S227A mutant driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)

Use

AAV

Tags

Flag

Promoter

CMV promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.CD86.C-HA

Plasmid

#154848

Purpose

Expresses murine CD86 protein (T-lymphocyte activation antigen) with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Cluster of Differentiation 86 (Cd86 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184V

Plasmid

#106190

Purpose

Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184V

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

Promoter

CAG

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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miR5 Mm ATP13A2

Plasmid

#171822

Purpose

transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

Depositor

Veerle Baekelandt

Article

van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

Insert

ATP13A2 (Atp13a2 Mouse)

Use

Lentiviral

Promoter

SFFV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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miR3 Mm ATP13A2

Plasmid

#171771

Purpose

transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

Depositor

Veerle Baekelandt

Article

van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

Insert

ATP13A2 (Atp13a2 Mouse)

Use

Lentiviral

Promoter

SFFV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC710 - pAAV EF1a DIO Mem-AcGFP

Plasmid

#75081

Purpose

An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

Insert

Mem-AcGFP

Use

AAV and Cre/Lox

Tags

Palmitylation site

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH

Plasmid

#112212

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

N-terminus of dCas9 and DNMT3A (DNMT3A Human)

Use

AAV

Mutation

H840A,N863A

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S1-jRCaMP1a

Plasmid

#160728

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S1-jRCaMP1a

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaTSR3-CTD-HA.SV40(polyA)

Plasmid

#155194

Purpose

Expresses truncated (AA 1-690) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Truncated sequence of THBS1 (expresses Amino Acid…

Promoter

CMV

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomArchon-mTagBFP2-p2A-CoChR-Kv2.1

Plasmid

#206006

Purpose

Expression of SomArchon-mTagBFP2 and CoChR under Syn promoter

Depositor

Xue Han

Article

Lowet et al Cell Rep. 2023 Aug 29;42(8):112906. doi:

Insert

SomArchon-mTagBFP2-P2A-CoChR-Kv2.1

Use

AAV

Tags

mTagBFP2

Expression

Mammalian

Promoter

hSyn

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.SF-Venus-iGluSnFR.S72A

Plasmid

#106179

Purpose

Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.S72A

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

Promoter

hSynapsin

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97310

Purpose

NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb NHEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLPX-rc [ChrimsonR-GFP]

Plasmid

#118295

Purpose

AAV-mediated expression of ChrimsonR-GFP under the CAG promoter in frt/reversed (Flp-dependent) manner.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

CAG

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAav-MDM2-PQS2-3xHA

Plasmid

#84886

Purpose

rAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Inserts

MDM2 genomic sequence HR1 (MDM2 Human)
MDM2 genomic insert HR2 (MDM2 Human)

Use

AAV

Tags

PQS2 3xHA

Promoter

no

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-Chronos-GFP

Plasmid

#122098

Purpose

AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1α (1.1 kb short version)

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NLS-IRES-hrGFP

Plasmid

#107543

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NLS (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-ChR2-YFP

Plasmid

#178725

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of ChR2-YFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

ChR2-YFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pQC MCS IRES G418

Plasmid

#110344

Purpose

γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Geneticin selection.

Depositor

Sandra Martha Gomes Dias

Article

Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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