164,890 results

1. SPLICS PM-ER Long P2A

   Plasmid

   #164111

   Purpose

   Detect the long Plasma membrane-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP PM-ER Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSynapsin-FLEX-soCoChR-GFP (AAV9)

   Viral Prep

   #107712-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSynapsin-FLEX-soCoChR-GFP (#107712). In addition to the viral particles, you will also receive purified pAAV-hSynapsin-FLEX-soCoChR-GFP plasmid DNA. Synapsin-driven, Cre-dependent somatic CoChR (soCoChR) fused to GFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Promoter

   Syn

   Tags

   GFP (Cre-dependent)

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

3. sox10:mCherry

   Plasmid

   #98695

   Purpose

   I-SceI meganuclease-mediated mCherry transgenesis in zebrafish

   Depositor

   Thomas Look

   Article

   Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

   Insert

   mCherry
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRY2olig-PR

   Plasmid

   #218917

   Purpose

   Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequence

   Depositor

   Tatyana Shelkovnikova

   Article

   Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

   Insert

   CRY2olig-mCherry tagged poly-PR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CRY2olig-GR

   Plasmid

   #218919

   Purpose

   Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequence

   Depositor

   Tatyana Shelkovnikova

   Article

   Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

   Insert

   CRY2olig-mCherry tagged poly-GR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET29a-human histone 4-in-One

   Plasmid

   #223237

   Purpose

   Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

   Depositor

   Weixing Zhao

   Inserts

   His6-VSVG-Histone H2A
   Histone H2B
   Histone H3
   Histone H4-TEV-His6-Dual StrepII
   

   Use

   Tags

   Dual StrepII, His6, TEV protease site, and VSVG

   Expression

   Bacterial

   Mutation

   A103G

   Promoter

   T7

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pILGFP91

   Plasmid

   #83548

   Purpose

   Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   PGK1 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK1

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMCB306-GFP-Rab8A

   Plasmid

   #198470

   Purpose

   lentiviral expression of GFP-Rab8A

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   GFP-Rab8A (RAB8A Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GAG-Pan

   Plasmid

   #99640

   Purpose

   EGFP-mCherry Met71GAG reporter for methionine misincorporation

   Depositor

   Tao Pan

   Article

   Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

   Insert

   mCherry
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Met71 mutated to GAG (Glu)

   Promoter

   CMV

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. T7-CMVtrans-FFLuc-polyA

    Plasmid

    #101156

    Purpose

    Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

    Depositor

    Marcel Bruchez

    Article

    Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. GAA-Pan

    Plasmid

    #99639

    Purpose

    EGFP-mCherry Met71GAA reporter for methionine misincorporation

    Depositor

    Tao Pan

    Article

    Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

    Insert

    mCherry
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Met71 mutated to GAA (Glu)

    Promoter

    CMV

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSC-GFP-NES-EGFP

    Plasmid

    #221810

    Purpose

    Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear export sequence (NES).

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    GFP-NES-EGFP
    

    Use

    Lentiviral

    Tags

    NES (internal tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-eGFP-Z1

    Plasmid

    #135049

    Purpose

    Enhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.

    Depositor

    Jordan Green

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-hSyn-mCherry-P2A-Cre-WPRE (AAV1)

    Viral Prep

    #107312-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-hSyn-mCherry-P2A-Cre-WPRE (#107312). In addition to the viral particles, you will also receive purified AAV-hSyn-mCherry-P2A-Cre-WPRE plasmid DNA. hSyn-driven expression of mCherry and Cre (physically separate). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Promoter

    Tags

    mCherry (physically separate, not a fusion protein)

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pLVX-Puro-TDP-43-A315T

    Plasmid

    #133755

    Purpose

    Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed alanine 315 to threonine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRUPATH Triple Ga12

    Plasmid

    #196061

    Purpose

    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PWPXLD-EGFR-WT

    Plasmid

    #133749

    Purpose

    Wild-type EGFR tagged with HA cloned into lentiviral PWPXLD plasimd

    Depositor

    Chay Kuo

    Article

    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

    Insert

    Wild type EGFR-HA (EGFR Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLIX402 PHF2

    Plasmid

    #91700

    Purpose

    inducible lentiviral expression of PHF2

    Depositor

    Bob Weinberg

    Article

    Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

    Insert

    PHF2 (PHF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1_FASN

    Plasmid

    #107138

    Purpose

    His tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cells

    Depositor

    Brijesh Kumar Singh

    Insert

    Fatty acid synthase (FASN Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSBbi-RP

    Plasmid

    #60513

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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