We narrowed to 19,003 results for: Tec

1. pGWB408

   Plasmid

   #74802

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT2-CAG-fGFP

   Plasmid

   #108714

   Purpose

   Transposon donor plasmid for long-term expression of membrane targetted GFP

   Depositor

   Madeline Lancaster

   Article

   Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666.

   Insert

   EGFP
   

   Tags

   6xHis and farnesylation signal

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-GfaABC1D-GRAB_eCB2.0

   Plasmid

   #164609

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in astrocytes

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Use

   AAV

   Mutation

   S383T

   Promoter

   GfaABC1D

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAsCpf1(TYCV)(BB) (pY211)

   Plasmid

   #89352

   Purpose

   Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K607R

   Promoter

   CBh

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCast

   Plasmid

   #200169

   Purpose

   E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

   Depositor

   Yong-Su Jin

   Article

   Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFEndoNA

   Plasmid

   #174724

   Purpose

   Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

   Depositor

   Jukka Finne

   Article

   Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

   Insert

   GFP-endoNA fusion protein
   

   Tags

   Histag

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBK-EQR-BE3

   Plasmid

   #85172

   Purpose

   Expresses EQR-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   EQR-BE3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPE3-pPuro

   Plasmid

   #215722

   Purpose

   Cloning vector for Uni-Vector prime editing system

   Depositor

   Kou-Juey Wu

   Article

   Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

   Insert

   PE2
   

   Use

   Unspecified

   Promoter

   EFS promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB409

   Plasmid

   #74803

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with 6xHis (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-c/o NL4-3 Env delta CT

    Plasmid

    #139285

    Purpose

    HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    codon optimized HIV-1 Env delta cytoplasmic tail
    

    Expression

    Mammalian

    Mutation

    deletion of cytoplasmic tail after residue 710

    Promoter

    CMV IE

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-Sp-gRNA-ALB_DF_B358c_attB

    Plasmid

    #182146

    Purpose

    To insert attB attachment site at ALB in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    ALB_B358c_attB pegRNA
    

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-Sp-gRNA-ALB_DF_A277c_attB

    Plasmid

    #182145

    Purpose

    To insert attB attachment site at ALB in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    Alb_A277c_attB pegRNA
    

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. p4A8_S7T_BC

    Plasmid

    #217832

    Purpose

    4A8 variant in barcoded, yeast surface display plasmid

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Insert

    4A8 variant Fab
    

    Expression

    Yeast

    Mutation

    VL: S7T

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB509

    Plasmid

    #74851

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 6xHis (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA FLAG TORC3

    Plasmid

    #22976

    Depositor

    Marc Montminy

    Article

    Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

    Insert

    TORC3 (CRTC3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pSL2669 (pTnsABf_Vch)

    Plasmid

    #200897

    Purpose

    Expresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchTnsA-TnsB
    

    Tags

    Internal NLS between TnsA-TnsB fusion

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. KG#901

    Plasmid

    #110936

    Purpose

    Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

    Inserts

    mig-13 promoter
    Emerald-RAB-3
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Expression

    Bacterial

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGWB545

    Plasmid

    #74879

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRN110

    Plasmid

    #127228

    Purpose

    CmYLCV::STM::35S terminator

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    STM (STM A. tumefaciens, Mustard Weed)
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTpal-fdc

    Plasmid

    #78288

    Purpose

    Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

    Depositor

    David Nielsen

    Article

    McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

    Inserts

    PAL2 (PAL2 Mustard Weed)
    FDC1 (FDC1 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    *see below

    Promoter

    trc

    Available Since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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