We narrowed to 11,028 results for: HA

1. pRK5 GST MetaP2

   Plasmid

   #112750

   Purpose

   express HA-GST-FRB-MetaP2

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   HA-GST-FRB-MetaP2 (METAP2 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLCHKO_SFRS7_exon_deletion_3

   Plasmid

   #155071

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLCHKO_MDM4_exon_deletion_3

   Plasmid

   #155075

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLCHKO_HPRT1_exon3_2_Lb

   Plasmid

   #155054

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 3
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTWIN1-His6-Ssp-Httex1-37Q

   Plasmid

   #84351

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 37Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mApple-M1 SpastinHFDD

   Plasmid

   #134462

   Purpose

   M1 SpastinHFDD expression

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

   Insert

   M1 Spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   Met to Ala at amino acid 87; His to Asp at amino …

   Promoter

   CMV

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

   Plasmid

   #119864

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Tags

   HA-tagged biotin ligase (BirA), Thosea asigna vir…

   Expression

   Bacterial

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RALYL-1

   Plasmid

   #155877

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   RALYL (RALYL Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLCMV:ECFP(loxP)(FRT)HNF1B

   Plasmid

   #31439

   Depositor

   Gerhart Ryffel

   Article

   Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Ma

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Cre/Lox; Flp/frt

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-B2UP49_GFP

    Plasmid

    #103071

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    B2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTWIN1-His6-Ssp-Httex1-15Q

    Plasmid

    #84348

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 15Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC19-A0LWB3

    Plasmid

    #103070

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    A0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Topo_SpCas9.tracr_LbCas12a.DR

    Plasmid

    #155049

    Purpose

    TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
    

    Use

    CRISPR

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3.1-nAChr-beta3

    Plasmid

    #48811

    Purpose

    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

    Depositor

    Jerry Stitzel, Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    

    Expression

    Mammalian

    Mutation

    *

    Promoter

    CMV

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC19-K1LM04

    Plasmid

    #103119

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    K1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80065

    Purpose

    Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Zic2a enhancer driving c-fos promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMSCV_PIG_3XHA-Ago1

    Plasmid

    #170916

    Purpose

    Expresses 3X-HA-AGO1

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    Ago1 (Ago1 Mouse)
    

    Tags

    3X-HA

    Expression

    Mammalian

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. P6601 MSCV-IP N-HAonly GARS

    Plasmid

    #34899

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    GARS (GARS1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCGN-HCF-1-N450

    Plasmid

    #92321

    Purpose

    N-term 1-450 of HCF-1 protein

    Depositor

    Winship Herr

    Article

    Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

    Insert

    HCF-1 (HCFC1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AA 2-450. Note that a SRRASGTYGRV amino-terminal …

    Promoter

    CMV

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-E6I4Q5

    Plasmid

    #103101

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E6I4Q5(Cas9 coding gene from Enterococcus faecalis TX0012)
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge