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pHCMV_CHIKV_E1E2

Plasmid

#207261

Purpose

Mammalian expression of Chikungunya virus E1E2 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

CHIKV_E1E2 (CHIKVgp2 Chikungunya virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Miro1v4

Plasmid

#174111

Purpose

Expresses the peroxisomal variant (splice variant 4) of Miro with an N-terminal EGFP.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Miro1 (var4, peroxisomal splice variant)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-TRBP

Plasmid

#15666

Depositor

Dong-Yan Jin

Article

Kok et al J Biol Chem. 2007 Jun 15. 282(24):17649-

Insert

TRBP (TARBP2 Human)

Expression

Mammalian

Available Since

Sept. 19, 2007

Availability

Academic Institutions and Nonprofits only
pHCMV_FLUBV_HA

Plasmid

#207274

Purpose

Mammalian expression of Influenza B virus HA protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

FLUBV_HA (HA Influenza B virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Cdc42

Plasmid

#180577

Purpose

ddGFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Cdc42

Expression

Mammalian

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pPD304 VPR-ZF43

Plasmid

#138840

Purpose

Constitutive expression of VPR-ZF43 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-05

Plasmid

#119984

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTagRFP675-N1

Plasmid

#44275

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

Insert

TagRFP675

Expression

Mammalian

Mutation

M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

Promoter

CMV

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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Epsin2-pmCherryC1

Plasmid

#27673

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Epsin 2 (Epn2 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (BB)-2A-GFP gamma-tubulin sg

Plasmid

#104437

Purpose

Knockdown the expression of gamma-tubulin in human cells

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin sgRNA

Tags

The gamma-tubulin sgRNA is coexpressed with 3xFLA…

Expression

Mammalian

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR4-myc-nuc (w556-1)

Plasmid

#20026

Purpose

Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway entry vector

Tags

Myc and NLS

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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mtagBFP2-CaM-uTEVp

Plasmid

#163033

Purpose

FLiCRE protease for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

BFP-CaM-uTEVp

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-PACT

Plasmid

#15667

Depositor

Dong-Yan Jin

Article

Kok et al J Biol Chem. 2007 Jun 15. 282(24):17649-

Insert

PACT (PRKRA Human)

Expression

Mammalian

Available Since

Sept. 19, 2007

Availability

Academic Institutions and Nonprofits only
726-Desmoglein2-myc

Plasmid

#32232

Depositor

Kathleen Green

Article

Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35

Insert

Desmoglein2 (DSG2 Human)

Tags

Myc

Expression

Mammalian

Promoter

human beta-actin

Available Since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only
PAGFP-Ub

Plasmid

#11933

Depositor

Nico Dantuma

Article

Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

Insert

Ubiquitin (RBX1 Human)

Tags

PAGFP

Expression

Mammalian

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pPD547 ZF43x6-Compact_EYFP

Plasmid

#138861

Purpose

EYFP reporter vector for the ZF43x6-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Compact

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTK296

Plasmid

#59565

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP-PEST

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn Kozak EGFP-PEST

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pEF HaloTag-SYT1

Plasmid

#197280

Purpose

Encodes full-length wild type synaptotagmin 1 (SYT1) with an N-terminal HaloTag

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYT1 and HaloTag (Syt1 Rat)

Expression

Mammalian

Promoter

EF1alpha

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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PKCalpha.FLAG

Plasmid

#10805

Depositor

Alex Toker

Article

Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

Insert

PKC alpha (PRKCA Bovine)

Tags

FLAG

Expression

Mammalian

Mutation

D332G-please see comments below

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.0

Plasmid

#208301

Purpose

Expresses ABE-RA3.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.0-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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