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pEF HaloTag-SYT1

Plasmid

#197280

Purpose

Encodes full-length wild type synaptotagmin 1 (SYT1) with an N-terminal HaloTag

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYT1 and HaloTag (Syt1 Rat)

Expression

Mammalian

Promoter

EF1alpha

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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PKCalpha.FLAG

Plasmid

#10805

Depositor

Alex Toker

Article

Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

Insert

PKC alpha (PRKCA Bovine)

Tags

FLAG

Expression

Mammalian

Mutation

D332G-please see comments below

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.0

Plasmid

#208301

Purpose

Expresses ABE-RA3.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.0-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_MeltCasp1-37-GFP

Plasmid

#234069

Purpose

Encodes the caspase1 without CARD domain (with R391K mutation) controlled by a Melt variant with a switching temperature of 37°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltCasp1-37-GFP

Use

Lentiviral

Expression

Mammalian

Mutation

Melt(C292A,Q355N); hcaspase1(R391K)

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV Sport2 mPer1

Plasmid

#16203

Depositor

Cheng Lee

Article

Albrecht et al Cell. 1997 Dec 26. 91(7):1055-64.

Insert

Per1 (Per1 Mouse)

Expression

Mammalian

Available Since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only
mNeonGreen-MapTau

Plasmid

#137808

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

MapTau

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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vYM140_PB-pEFa1-iaaM(P.s)-Hygro

Plasmid

#160043

Purpose

Expressing iaaM for converting L-Trp to IAM

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Insert

iaaM (iaaM )

Use

Synthetic Biology

Expression

Mammalian

Promoter

pEF1a

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pL-rpb1-N488D

Plasmid

#10988

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-N488D (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 1462 A to G in the RPB1 ORF resulting in…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
p178 Grg-1L Myc His

Plasmid

#11067

Depositor

Ramesh Shivdasani

Article

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-L (Tle1 Mouse)

Tags

His and Myc

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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SM282_EF1A-dEnAsCas12a-P300-T2A-BlastR

Plasmid

#244217

Purpose

Mammalian expression of dEnAsCas12a-P300 with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR4-HA-myc-nuc (w557-1)

Plasmid

#20025

Purpose

Gateway entry vector for nuclear targeting of domains (N-term HA , C-term NLS, myc tags)

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

HA, Myc, and NLS

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pCLE NICD3

Plasmid

#26894

Depositor

Nicholas Gaiano

Article

Dang et al Dev Neurosci. 2006;28(1-2):58-69.

Insert

NICD3 (NOTCH3 Human)

Use

Retroviral

Tags

Alkaline Phosphatase and IRES

Expression

Mammalian

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pME-minP:luc2

Plasmid

#138354

Purpose

Gateway compatible middle entry clone contains Firefly Luciferase2 insert driven by minimal promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

Luciferase

Expression

Mammalian

Promoter

minimal promoter

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHCMV_POWV_PrME

Plasmid

#207299

Purpose

Mammalian expression of Tick-borne powassan virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

POWV_PrME (POLY Tick-borne powassan virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA6 MCV LTco

Plasmid

#40200

Depositor

Patrick Moore

Article

Shuda et al J Clin Invest. 2011 Sep;121(9):3623-34.

Insert

MCPyV LT codon optimized

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-VCre

Plasmid

#89575

Purpose

Expresses VCre under pCAG

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

VCre recombinase

Use

Cre/Lox and Synthetic Biology

Tags

NLS

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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13xLexAop2 IVS sfGFP1-10

Plasmid

#221834

Purpose

Plasmid for generating 13xLexAop2 IVS sfGFP1-10 with attP/attB integration. It carries the attB sequence.

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

sfGFP1-10

Expression

Insect

Promoter

hsp70

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-tdMCP-msfGFP(V206K)-GBI

Plasmid

#205163

Purpose

express tdMCP-msfGFP(V206K)-GBI

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

tdMCP-msfGFP(V206K)-GBI

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pZ149-pcDNA3-BhCas12b_v4

Plasmid

#122446

Purpose

Expresses BhCas12b_v4 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BhCas12b_v4-NLS-3xHA

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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Tad-CBE2.4

Plasmid

#235055

Purpose

Expresses Tad-CBE2.4 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE2.4-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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