We narrowed to 24,922 results for: Spr

1. RPS6KA3 gRNA (BRDN0001148452)

   Plasmid

   #75943

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RPS6KA3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RPS6KA3 (RPS6KA3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTE4497

   Plasmid

   #80339

   Purpose

   mammalian expression FnCpf1 nuclease and Fn crRNA

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   FnCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Promoter

   CMV and human U6

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLC-BFP-FADD

   Plasmid

   #75166

   Purpose

   LentiCRISPR-BFP with sgRNA targeting human FADD

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Insert

   FADD sgRNA (FADD Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pROS11

   Plasmid

   #107925

   Purpose

   amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RPS6KA3 gRNA (BRDN0001147852)

   Plasmid

   #75944

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RPS6KA3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RPS6KA3 (RPS6KA3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82702

   Purpose

   AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   H1/TO Empty gRNA Expression Cassette
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA

   Plasmid

   #113686

   Purpose

   An EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   de-catalyzed SaCas9
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   KRAB

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CSNK1G2 gRNA (BRDN0001162501)

   Plasmid

   #76988

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CSNK1G2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CSNK1G2 (CSNK1G2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CSNK1G2 gRNA (BRDN0001149101)

   Plasmid

   #76989

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CSNK1G2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CSNK1G2 (CSNK1G2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CSNK1G2 gRNA (BRDN0001145026)

    Plasmid

    #76990

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CSNK1G2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CSNK1G2 (CSNK1G2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. WT1 KI gRNA2

    Plasmid

    #92313

    Purpose

    CRISPR-GFP-gRNA for cutting WT1

    Depositor

    Sean Palecek

    Article

    Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

    Insert

    WT1 (WT1 Human)
    

    Tags

    CRISPR GFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PB-PE xCas9

    Plasmid

    #172652

    Purpose

    All-in-one prime editor piggyBac transposon (xCas9)

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

    Plasmid

    #136660

    Purpose

    Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    ABE7.10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS and hSt1Cas9

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CD2 gRNA (BRDN0001146864)

    Plasmid

    #75827

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CD2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CD2 (CD2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

    Plasmid

    #68345

    Purpose

    A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

    Inserts

    puromycin
    hCas9
    

    Use

    CRISPR; Flp/frt

    Tags

    linked to red flouresent protein gene mKateII and…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTE4889

    Plasmid

    #88905

    Purpose

    Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Fn crRNA
    hFnCpf1(D917A)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Promoter

    CMV and human U6

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CSK gRNA (BRDN0001149071)

    Plasmid

    #77768

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CSK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CSK (CSK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pROS16

    Plasmid

    #107930

    Purpose

    HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CDK2 gRNA (BRDN0001148866)

    Plasmid

    #77191

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK2 (CDK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GSK3B gRNA (BRDN0001145264)

    Plasmid

    #77281

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GSK3B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    GSK3B (GSK3B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge