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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 26,332 results for: LEA

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Showing: 6101 - 6120 of 26332 results

  1. pHS3

    Plasmid
    #81038
    Purpose
    HO endonuclease expression
    Depositor
    John McCusker
    Insert
    NATMX - HO endonuclease
    Expression
    Yeast
    Promoter
    TEF
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  8. GST-BHMT-I/G

    Plasmid
    #104442
    Purpose
    Autophagy cargo assay
    Depositor
    Carol Mercer
    Publication
    Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BHMT (BHMT Human)
    Tags
    GFP-myc GFP is from Aequorea victoria and GST
    Expression
    Mammalian
    Mutation
    N43A in GST (in a potential KFERQ-like sequence).…
    Promoter
    CMV
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104442-plasmid-map-sequence-id-200428

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  11. Tol2-CAG::Mitbow

    Plasmid
    #158994
    Purpose
    Multicolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Publication
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Mitbow
    Tags
    The default expressed EBFP2 is fused to H2B; oth…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158994-plasmid-map-sequence-id-311800

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  15. TUBG1

    Plasmid
    #87954
    Purpose
    expresses human gamma-tubulin with a GFP-tag on the N-terminal of the protein
    Depositor
    Maria Alvarado-Kristensson
    Publication
    Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gamma-tubulin 1 (TUBG1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    whole protein, The C-terminal is a sh-gamma-tubul…
    Available since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    87954-plasmid-map-sequence-id-178205

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  16. pMT-sox10-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80062
    Purpose
    Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Sox10 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80062-plasmid-map-sequence-id-417618

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  17. pRR-AR

    Plasmid
    #73045
    Purpose
    Constitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)
    Depositor
    David McMillen
    Publication
    Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-
    Insert
    Androgen receptor (AR Human)
    Expression
    Yeast
    Mutation
    please see depositor comments below
    Promoter
    glyceraldehyde phosphate dehydrogenase (GPD)
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    73045-plasmid-map-sequence-id-134099

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  18. pOCC304

    Plasmid
    #118897
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with TEVprotease and TwinStrep-MB…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118897-plasmid-map-sequence-id-232369

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  19. pcDNA3-TRAIL-R3

    Plasmid
    #61384
    Purpose
    Expresses TRAIL receptor 3
    Depositor
    Francis Chan
    Publication
    Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TRAIL receptor 3 (TNFRSF10C Human)
    Tags
    HA tag and TNF receptor leader peptide
    Expression
    Mammalian
    Mutation
    TRAIL-R3 leader peptide (aa1-29) replaced with TN…
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. TALEN-CCR5L18-CtermQ7

    Plasmid
    #51442
    Purpose
    Expresses TALEN targeting left CCR5A site (L18) with Q7 C-terminal domain
    Depositor
    David Liu
    Publication
    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:
    Insert
    TALEN CCR5 Left (L18) Q7 C-term
    Use
    TALEN
    Tags
    3xFLAG and Fok1 EL variant
    Expression
    Mammalian
    Mutation
    K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q
    Promoter
    CMV
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51442-plasmid-map-sequence-id-78450

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Showing: 6101 - 6120 of 26332 results