We narrowed to 9,182 results for: mel

1. TRE-SRRD-mRuby3

   Plasmid

   #214920

   Purpose

   inducible expression of SRRD tagged on C-terminus with mRuby3

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   SRRD (SRRD Human)
   

   Use

   Lentiviral

   Tags

   mRuby3

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a-SRRD-noTag (in pLEX_307)

   Plasmid

   #214919

   Purpose

   constitutive expression of SRRD with no tag - used as control for SRRD-HA expression

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   SRRD (SRRD Human)
   

   Use

   Lentiviral

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1a-SRRD-HA (in pLEX_307)

   Plasmid

   #214918

   Purpose

   constitutive expression of SRRD tagged with HA

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   SRRD (SRRD Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Amber-free HIV-1 deltaRT

   Plasmid

   #213007

   Purpose

   An replication-incompetent amber-free HIV-1 encodes full-length Env BG505 with RT-deleted Q23 as the backbone.

   Depositor

   Maolin Lu

   Article

   Ao et al Cell Chem Biol. 2024 Jan 8:S2451-9456(23

   Inserts

   HIV-1 Q23 ΔRT backbone (reverse transcriptase is deleted)
   Env BG505
   

   Use

   Unspecified

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. YW347_Ptbb2UTR-loxP-RFP-miniMOS

   Plasmid

   #196064

   Purpose

   a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.

   Depositor

   Wenxing Yang

   Article

   Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

   Inserts

   myo2p driven tagRFP
   NeoR
   Minimal Mos1
   

   Expression

   Worm

   Promoter

   myo-2

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPBbleo-miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

   Plasmid

   #186703

   Purpose

   Mammalian expression of Tiam1(DH-PH) fused to miRFP703-eDHFR(69K6)

   Depositor

   Michiyuki Matsuda

   Article

   Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

   Insert

   miRFP703-eDHFR(69K6)-Tiam1(DH-PH)
   

   Expression

   Mammalian

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNHT7::UMSBP::sNluc2_ORF2

   Plasmid

   #186766

   Purpose

   An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon..

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-Duet1-6xHis-TEV-OPTN

   Plasmid

   #190192

   Purpose

   Plasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397

   Depositor

   Sascha Martens

   Article

   Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   optineurin (OPTN Human)
   

   Tags

   6xHis and TEV

   Expression

   Bacterial

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9_nonPuro_OSS

   Plasmid

   #186652

   Purpose

   Cas9 plasmid to express the Cas9 in OSS cells

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Cas9 Expression Plasmid
   

   Expression

   Insect

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pESC-LEU-IpgD

    Plasmid

    #183672

    Purpose

    Inducible Shigella flexneri IpgD for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-anti-MIR328-3p

    Plasmid

    #153319

    Purpose

    Expresses zsGreen fused to a miR-328-3p sponge and mCherry in mammalian cells.

    Depositor

    Robert Judson-Torres

    Article

    McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

    Insert

    zsGreen with MIR328-3p sponge
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-anti-MIR211-5p

    Plasmid

    #153318

    Purpose

    Expresses zsGreen fused to a miR-211-5p sponge and mCherry in mammalian cells.

    Depositor

    Robert Judson-Torres

    Article

    McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

    Insert

    zsGreen with MIR211-5p sponge
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSJ982

    Plasmid

    #176622

    Purpose

    Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

    Depositor

    Alexander Varshavsky

    Article

    Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

    Insert

    Ub-MPGLW-Fbp1
    

    Tags

    Ubiquitin N-terminal fusion and triple flag tag C…

    Expression

    Yeast

    Mutation

    Changed first 4 N-terminal amino acid residues of…

    Promoter

    TDH3tc3

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSIN-Gapdh

    Plasmid

    #175938

    Purpose

    knockout mouse Gapdh

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Gapdh gRNA (Gapdh Mouse)
    

    Use

    Retroviral

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-3xFLAG-Puro PAK5 K478R/479R S364L

    Plasmid

    #167199

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R S364L; PAK7 Kinase dead & S364L double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R S364L
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGG198

    Plasmid

    #165605

    Purpose

    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFP

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Secondary protospacer ('CGGCG' PAM) ins…

    Promoter

    lac

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-3xFLAG-Puro PAK5 K478R/479R D421N

    Plasmid

    #167200

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R D421N
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-3xFLAG-Puro PAK5 K478R/479R E294K

    Plasmid

    #167198

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R E294K
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-3xFLAG-Puro PAK5 K478R/479R M173I

    Plasmid

    #167197

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R M173I; PAK7 Kinase dead & M173I double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R M173I
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-3xFLAG-Puro PAK5 K478R/479R E144K

    Plasmid

    #167196

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E144K; PAK7 Kinase dead & E144K double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R E144K
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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