Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 23,173 results for: LEA

Clear keywords
Showing: 6121 - 6140 of 23173 results

  4. p5T7-LYCipi-ggpps

    Plasmid
    #122017
    Purpose
    Expresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.
    Depositor
    Gregory Stephanopoulos
    Publication
    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p
    Insert
    GGPP synthase
    Expression
    Bacterial
    Mutation
    codon optimized, truncated first 98 amino acids, …
    Promoter
    T7
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    122017-plasmid-map-sequence-id-238964

    Enlarge

  5. zCD2

    Plasmid
    #122031
    Purpose
    Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.
    Depositor
    David Christianson
    Publication
    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi
    Insert
    Zebrafish HDAC6 Catalytic Domain 2
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122031-plasmid-map-sequence-id-240596

    Enlarge

  6. pCMV-BLZinCh-1

    Plasmid
    #85082
    Purpose
    mammalian vector for the expression of a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging
    Depositor
    Maarten Merkx
    Publication
    Aper et al ACS Chem Biol. 2016 Oct 21;11(10):2854-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BL-ZinCh-1
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    85082-plasmid-map-sequence-id-164057

    Enlarge

  9. EGFR-ET1

    Plasmid
    #44185
    Depositor
    Timothy Springer
    Publication
    Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFR-ET1 (EGFR Human)
    Tags
    3C cleavge site, protein C peptide tag, his6, SBP…
    Expression
    Mammalian
    Mutation
    I104V and D254N relative to NP_005219.2
    Promoter
    CMV
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    44185-plasmid-map-sequence-id-458206

    Enlarge

  11. pFastBac1-His10FLAG

    Plasmid
    #157736
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Publication
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Tags
    His10 + 3xFLAG, enterokinase-cleavable
    Expression
    Insect
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    157736-plasmid-map-sequence-id-333926

    Enlarge

  12. UBH14

    Plasmid
    #28158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    E3 ligase UHRF2 (UHRF2 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    SRA Domain (residues 419-648)
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pAAV-EF1a-vCre (AAV8)

    Viral prep
    #55638-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-EF1a-vCre (#55638). In addition to the viral particles, you will also receive purified pAAV-EF1a-vCre plasmid DNA. EF1a-driven expression of VCre. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Available since
    Nov. 12, 2020
    Availability
    Academic Institutions and Nonprofits only

  16. pOCC72

    Plasmid
    #118866
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric EGFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mEGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, mEGFP, cleavabl…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118866-plasmid-map-sequence-id-232323

    Enlarge

  17. NCOR2

    Plasmid
    #40737
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NCOR2 (NCOR2 Human)
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Residues 615 to 685 of the SANT2 domain of Nuclea…
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 6121 - 6140 of 23173 results