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Plasmid#120163PurposeExpresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertkinesin family member 5A (Kif5a Mouse)
TagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIF5A: 1-559 aaPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-SBP-VAP-A*
Plasmid#120175PurposeExpresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-ADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVAMP associated protein A (VAPA Human)
TagsEGFP and SBPExpressionMammalianMutationTruncated VAP-A: 118-249PromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACT1:Cas9-GFP, U6:sgUPRT
Plasmid#122853PurposeExpresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9-GFP
U6-sgUPRT
UseCRISPR; Cas9-gfp plasmid for genome editing of cr…TagseGFPPromoterCryptosporidium parvum U6 and Cryptosporidium par…Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA-CXCR4(90)
Plasmid#98962PurposeMammalian expression plasmid for CRISPR gRNA targeting human CXCR4 exon 2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCXCR4 (CXCR4 Human)
UseCRISPRExpressionMammalianMutationEncodes a gRNA for CRISPR-mediated targeting of C…PromoterU6Available sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
U6-hGRIN2B-CAG-ps-SaCas9
Plasmid#102853PurposeA single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B Human, S. aureus, Synthetic)
UseCRISPRTags3X Flag and NLSExpressionMammalianPromoterU6 promoter;Available sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
hNTa1-qgRNA-pYJA5
Plasmid#217779PurposeNon-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloningDepositorInsertQuadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
UseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianPromoterhuman U6, mouse U6, human H1, human 7SKAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
delCMV-mCherry-3x-p67phox-GBD
Plasmid#214141PurposeRac activity sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xp67phox-GBD (NCF2 Human)
TagsmCherryExpressionMammalianMutationOnly the GTPase binding domain (GBD) is includedPromoterdelCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-HSV TK-P2A-H2-Kb SCT-T2A-mCD47-E2A-mCD55-F2A-neo-AAVS1
Plasmid#205446Purposehuman AAVS1-targeting plasmid for expression of HSV TK, H2-Kb SCT, mouse CD47, and mouse CD55DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHSV TK-H2-Kb SCT-mCD47-mCD55
ExpressionMammalianPromoterEF1aAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
MF83_PB-TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
Plasmid#176627PurposeTF B self-activation cassette in MultiFate-2.2 and MultiFate-3DepositorInsertTRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
UseSynthetic BiologyAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV_iRFP_P2AT2A_mScarlet-I_Myl9
Plasmid#172474PurposeMammalian expression of myosin light chain (Myl9) tagged with mScarlet-I and cytoplasmic iRFP as a reference marker.DepositorInsertsUseLentiviralTagsmScarlet-IExpressionMammalianPromoterEF1alpha (with UCOE) and none (same transcript as…Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-SpRY-P2A-mScarlet (MNW002)
Plasmid#174137PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarletDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationSpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…PromoterCMV and T7Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-Usp9x_CD-mCherry
Plasmid#170380PurposeVector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUbiquitin specific protease 9x catalytic domain (Usp9x Mouse)
TagsmCherry-3xFlagExpressionMammalianMutationCatalytic domainAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)
Plasmid#139990PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationVQR=D1135V/R1335Q/T1337RPromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eA3A-BE3 (pRZ206)
Plasmid#131315PurposeCAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
ExpressionMammalianPromoterCAGAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-CFP-KRAS4B
Plasmid#112717PurposeExpresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCFP-KRAS4B (KRAS Human)
ExpressionMammalianPromoterCMVAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-endo-GsCT
Plasmid#205516PurposeEndosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertendo-GsCT (GNAS Human, Synthetic)
Tags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…ExpressionMammalianPromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
RUNX1_pcDNA6.2/EmGFP-Bsd
Plasmid#176974PurposeMammalian expression vector encoding RUNX1 and EmGFP-BsdDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRUNX1 (RUNX1 Human)
ExpressionMammalianPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)
Plasmid#169191PurposeTo express nsp10-nsp16 fusion protein in E. coliDepositorInsert14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab SARS-CoV-2, Synthetic)
Tags14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)
Plasmid#169160PurposeTo express nsp14-nsp10 fusion protein in E. coliDepositorInsert14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab SARS-CoV-2, Synthetic)
Tags14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin
Plasmid#187953PurposeFKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFKBP12_F36V-SpdCas9-KRAB-tagBFP
UseCRISPR and Synthetic BiologyTagsGGSExpressionMammalianAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only