We narrowed to 11,551 results for: ANG

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Anti-VAPA [N479/12R]

Plasmid

#199401

Purpose

Mammalian expression plasmid of Anti-VAPA [N479/12R] (Rat). Derived from hybridoma N479/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-VAPA (Rat) recombinant mouse monoclonal antibody (Vapa Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[V458A][Q459A][V460A][G461A]-FLAG

Plasmid

#199328

Purpose

mutated RHIM motive (interaction inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed V458, Q459,V460 and G461 to alanine

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS

Plasmid

#194755

Purpose

Lentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchor

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Insert

PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS

Use

Lentiviral

Tags

HA

Promoter

CMV

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-353)GCN4-L-GFP

Plasmid

#193714

Purpose

Expression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-353)-GCN4-L-GFP (KIF5B Synthetic, Human)

Tags

EGFP

Expression

Mammalian

Mutation

hsKIF5B(aa1-353);

Promoter

Chicken beta-actin

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-SAP102 [N19/2R]

Plasmid

#177510

Purpose

Mammalian Expression Plasmid of anti-SAP102 (Rat). Derived from hybridoma N19/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-SAP102 (Rattus norvegicus) recombinant mouse monoclonal antibody (Dlg3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-NALCN [N185/7R]

Plasmid

#177508

Purpose

Mammalian Expression Plasmid of anti-NALCN (Rat). Derived from hybridoma N185/7.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NALCN (Rattus norvegicus) recombinant mouse monoclonal antibody (Nalcn Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG_FBXL7 (R310H)

Plasmid

#171849

Purpose

pcDNA3-FLAG_FBXL7 (R310H)

Depositor

Michele Pagano

Article

Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

Insert

F-box and leucine rich repeat protein 7 (FBXL7 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Arginine 310 to Histidine

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant

Plasmid

#173963

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif

Use

Luciferase

Mutation

1 mutated Coordinator motif

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

Plasmid

#158952

Purpose

Express human RAD51(F129A) mutant in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

RAD51 (RAD51 Human)

Tags

2X FLAG 2X STREP

Expression

Mammalian

Mutation

changed phenylalanine 129 to alanine

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3746

Plasmid

#154109

Purpose

loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::His-58::FRT3
mex-5p::FLP D5::sl2::mNG::glh2 3'

Use

Cre/Lox; Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCI.neo-rPAM1-M314I

Plasmid

#145802

Purpose

expresses catalytically inactive rat PAM-1 in mammalian cells

Depositor

Betty Eipper, Richard Mains

Article

Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

Insert

rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)

Expression

Mammalian

Mutation

changed methionine 314 to isoleucine to inactivat…

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-(BsaI)_CBh-Cas9

Plasmid

#135010

Purpose

Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

Depositor

Yosef Shaul

Article

Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

Insert

humanized S. pyogenes Cas9

Tags

3xFLAG (N terminal on insert)

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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hKCNB1 L211P

Plasmid

#124487

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed leucine 211 to proline

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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hKCNB1 G381R

Plasmid

#124490

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed glycine at 381 to arginine

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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hKCNB1 F416L

Plasmid

#124491

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed phenyalanine 416 to leucine

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-V5H6-mPCSK3 D153A

Plasmid

#122681

Purpose

Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)

Expression

Mammalian

Mutation

changed Aspartic Acid 153 to Alanine

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-BirA-eCPX-AP(K10)

Plasmid

#121908

Purpose

Expresses BirA and eCPX-AP(K10)

Depositor

Per Svenningsen

Article

Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

Inserts

BirA (birA Synthetic)
enhanced Circularly Permuted Outer Membrane Protein X

Tags

6xHis

Expression

Bacterial

Mutation

changed lysine 10 in the AP to alanine

Promoter

T7 and pBAD promoter

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 T134R,C91S-pcDNA3.1

Plasmid

#118212

Purpose

Expresses FLAG-OTUB1 T134R,C91S in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

changed cysteine 91 to serine and threonine 134 t…

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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KG#205

Plasmid

#110881

Purpose

Expresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

Inserts

rab-3 promoter
pde-4d (isoform d) D448N cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Mutation

changed Asp 448 to Asn

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mCE(K294A)

Plasmid

#82461

Purpose

Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mCE (K294A) (Rngtt Mouse)

Tags

myc

Expression

Mammalian

Mutation

Lysine 294 changed to Alanine

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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