We narrowed to 1,926 results for: poli
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Plasmid#140844PurposeHis-tagged CsoS2 C-terminal domain (CTD), pET-14b backboneDepositorInsertCsoS2 C-terminal domain (CTD)
TagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 1-594PromoterT7Available SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA-dabAB2
Plasmid#133012PurposepFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes (locus_tag: BAS2959)DepositorInsertsdabB2
dabA2
ExpressionBacterialPromotertetRAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFE-dabAB2 C351A
Plasmid#133005PurposepFE carrying a dabA2 gene with a C351A mutation and a dabB2 gene (Uniprot: D0KWS8)DepositorInsertsdabB2
dabA2
ExpressionBacterialMutationC351APromotertetRAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a - MPX
Plasmid#89619Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertMitocondrial Tryparedoxin peroxidase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - EtfA
Plasmid#89616PurposeExpresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG inductionDepositorInsertElectron transport flavoprotein alpha subunit
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - EtfB
Plasmid#89617PurposeExpresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG inductionDepositorInsertelectron-transfer-flavoprotein, alpha polypeptide
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - TDR
Plasmid#89627PurposeExpresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG inductionDepositorInsertthiol dependent reductase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NFDD
Plasmid#89624PurposeExpresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG inductionDepositorInsertNAD/FAD dependent dehydrogenase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBP-J23108
Plasmid#72964PurposeLevel 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)DepositorInsertJ23108 promoter
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-J23114
Plasmid#72965PurposeLevel 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)DepositorInsertJ23114 promoter
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7_RBS
Plasmid#72978PurposeT7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)DepositorInsertT7+RBS
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-lacZ
Plasmid#72950PurposeLevel 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - A site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-B-lacZ
Plasmid#72951PurposeLevel 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - B site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-C-lacZ
Plasmid#72952PurposeLevel 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - C site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-D-lacZ
Plasmid#72953PurposeLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - D site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-B-RFP
Plasmid#72955PurposeLevel 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - B site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-C-RFP
Plasmid#72956PurposeLevel 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - C site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-D-RFP
Plasmid#72957PurposeLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - D site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-b-RFP
Plasmid#72959PurposeLevel 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABCDepositorInsertLevel 2 - b site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TCEB3B
Plasmid#67117PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 ZFYVE9
Plasmid#67134PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
SPLICS LY-MT Short P2A
Plasmid#213614PurposeDetect the short-range Lysosome-Mitochondria contactDepositorInsertSPLICS LY-MT Short P2A
ExpressionMammalianAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-GFP
Plasmid#78546PurposeFusion protein Cas9+GFPDepositorInsertGFP
UseLentiviralExpressionMammalianPromoterFusion protein with Cas9Available SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
SB100X in pCAG globin pA
Plasmid#127909PurposeSleeping Beauty Transposase expression vector driven by the CAG promoterDepositorInsertSleeping Beauty Transposase
ExpressionMammalianAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
SPLICS LY-MT Long P2A
Plasmid#213613PurposeDetect the long-range Lysosome-Mitochondria contactDepositorInsertSPLICS LY-MT Long P2A
ExpressionMammalianAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7_RBS-His6-Thrombin
Plasmid#72979PurposeT7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)DepositorInsertT7+RBS+His6+thrombin
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-T2A-GFP
Plasmid#78548PurposeCas9 protein with GFPDepositorInsertT2A-GFP
UseLentiviralExpressionMammalianPromoterIn frame with Cas9Available SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-BFP
Plasmid#78545PurposeFusion protein Cas9+BFPDepositorInsertBFP
UseLentiviralExpressionMammalianPromoterFusion protein with Cas9Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-T2A-BFP
Plasmid#78547PurposeCas9 protein with BFPDepositorInsertT2A-BFP
UseLentiviralExpressionMammalianPromoterIn frame with Cas9Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_IRES_tdTomato
Plasmid#184046PurposeExpresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequenceDepositorInsertsEnhanced Green Fluorescent Protein
tdTomato
Internal Ribosome Entry Site
ExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry
Plasmid#78534PurposeBackbone to clone single or paired sgRNAsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterU6 (for expressing sgRNA)Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYES1L-URA
Plasmid#84301PurposeE.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hFrataxin-HA
Plasmid#31895DepositorAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL6
Plasmid#67061PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CIRBP
Plasmid#67055PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-a (p15A origin)
Plasmid#74088PurposeSecondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
H1.224.M.luc
Plasmid#193705PurposeExpresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutatedDepositorInsertTruncated PolIII H1 promoter (H1.224) with the TATA box mutated
UseLuciferaseExpressionMammalianAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZDonor-Nm-Cas9-gRNA-hU6
Plasmid#61366Purposeempty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNADepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U6Available SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (p15A origin)
Plasmid#74090PurposepTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
UseSynthetic BiologyExpressionBacterialPromoterR0010 Const. Promoter controlling RFPAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GDI2
Plasmid#67093PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only