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Plasmid#166021PurposeExpresses mRuby in CaMKII+ cells with low miRNA-128 expressionDepositorInsertmRuby2 with 4 miR-128 binding sites
UseAAV and Mouse TargetingTagsNoneExpressionMammalianMutationNonePromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
GCN5 FL-dCas9-CBP core
Plasmid#179556Purposeencodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
GCN5 core-dCas9-CBP core
Plasmid#179555Purposeencodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
GCN5 FL-dCas9-p300 core
Plasmid#179554Purposeencodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)
Plasmid#188383PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Pax6(DBD)-p65(428-551)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACE2(-1119)-luc
Plasmid#31110PurposeLuciferase reporter construct for human ACE2 promoterDepositorInsertACE2 promoter (ACE2 Human)
UseLuciferaseTagsExpressionMammalianMutationpromoter region -1119 to +103PromoterAvailable sinceOct. 14, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (HNF1A)
Plasmid#188384PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)
Plasmid#188385PurposeLentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factorDepositorInsertPGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACE2(-252)-luc
Plasmid#31111PurposeLuciferase reporter construct for human ACE2 promoterDepositorInsertACE2 promoter (ACE2 Human)
UseLuciferaseTagsExpressionMammalianMutationpromoter region -252 to +103PromoterAvailable sinceOct. 14, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
ACE2(-202)-luc
Plasmid#31112PurposeLuciferase reporter construct for human ACE2 promoterDepositorInsertACE2 promoter (ACE2 Human)
UseLuciferaseTagsExpressionMammalianMutationpromoter region -202 to +103PromoterAvailable sinceOct. 14, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
PARD6A_PDZ_1
Plasmid#103946PurposeProtein expression and purification of TAX INTERACTION PROTEIN PDZ domainDepositorInsertPARD6A (PARD6A Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3 SIRT1-NTerm Exon1-Exon3
Plasmid#105681PurposeBacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2)DepositorInsertSIRT1-NTerm: Exon1-Exon3 (Sirt1 Mouse)
UseTagsGSTExpressionBacterialMutationSynonymous base changes not affecting the protein…Promotertac_PromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3
Plasmid#105680PurposeBacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3DepositorInsertSIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)
UseTagsGSTExpressionBacterialMutationSynonymous base changes not affecting the protein…Promotertac_PromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pECE-M2-PAK2 wt
Plasmid#31663DepositorInsertp21-activated protein kinase 2 (PAK2 Human)
UseTagsM2ExpressionMammalianMutationPromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PAK2 wt
Plasmid#31671DepositorInsertp21-activated protein kinase 2 (PAK2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
FUW-M2-PAK2 wt
Plasmid#31680DepositorInsertp21-activated protein kinase 2 (PAK2 Human)
UseLentiviralTagsM2ExpressionMammalianMutationPromoterAvailable sinceApril 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
Activin A (S2-2)
Plasmid#184621Purposefor transfection into S2 cells and expression of Activin A proteinDepositorInsertINHBA (INHBA Human)
UseTags8x His - streptavidin binding peptideExpressionInsectMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3 T2P 800
Plasmid#131147PurposeTo investigate activity of a 800 bp region upstream of mouse Trib2.DepositorInsert-800 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
UseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 T2P 2.6
Plasmid#131151PurposeTo investigate activity of a 2.6 kb region upstream of mouse Trib2.DepositorInsert-2.6kb - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
UseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 T2P 500
Plasmid#131150PurposeTo investigate activity of a 500 bp region upstream of mouse Trib2.DepositorInsert-500 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
UseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only