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We narrowed to 2,690 results for: BFP

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Showing: 601 - 620 of 2690 results

  1. mCherry_LD 0_CS G_tTA-BFP

    Plasmid
    #58856
    Purpose
    MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58856-plasmid-map-sequence-id-94228

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  3. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid
    #84244
    Purpose
    Expresses GA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    GAI-tagBFP-Sa dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available Since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    84244-plasmid-map-sequence-id-160887

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  4. pNLS-2xBFP(4C)

    Plasmid
    #176151
    Purpose
    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    NLS-2xBFP(4C)
    Tags
    6xHis tag and SV40 nuclear localization sequence …
    Expression
    Bacterial
    Mutation
    Compared with EGFP, the two BFP domains have the …
    Promoter
    Ptrc
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    176151-plasmid-map-sequence-id-356068

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  5. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118157
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118157-plasmid-map-sequence-id-240364

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  9. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

    Plasmid
    #84243
    Purpose
    Expresses ABA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    ABI-tagBFP-Sa dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available Since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    84243-plasmid-map-sequence-id-160884

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  11. dTomato_LD 0_CS G_tTA-BFP

    Plasmid
    #58871
    Purpose
    MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58871-plasmid-map-sequence-id-94232

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  15. pTRE-ICP4-BFP-Neo

    Plasmid
    #250609
    Purpose
    Plasmid vector expressing Tet-inducible codon-optimized HSV-1 ICP4 with a BFP reporter
    Depositor
    Liam Holt
    Inserts
    ICP4
    TagBFP2
    Expression
    Mammalian
    Mutation
    Codon-optimized for Homo sapiens
    Available Since
    May 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    250609-plasmid-map-sequence-id-508572

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  16. PB-TO-oNGN2

    Plasmid
    #198397
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…
    Promoter
    CAG, EF1a, and TRE3G
    Available Since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    198397-plasmid-map-sequence-id-389535

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  18. dTomato_LD 0_CS M_tTA-BFP

    Plasmid
    #58875
    Purpose
    MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58875-plasmid-map-sequence-id-94236

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  19. CD4_LD 0_CS G_tTA-BFP

    Plasmid
    #58869
    Purpose
    MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58869-plasmid-map-sequence-id-94230

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  20. PB-TO-hNIL

    Plasmid
    #172113
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Promoter
    EF1a and TRE3G
    Available Since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172113-plasmid-map-sequence-id-359519

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Showing: 601 - 620 of 2690 results