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p3A9cbetaTCR

Plasmid

#69600

Purpose

TCR beta chain transgene expression plasmid which has a unique ClaI site for the insertion of a TCR beta chain VDJ exon

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pKDR-CBh

Plasmid

#183557

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated CBh promoter)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Unattenuated

Expression

Mammalian

Promoter

CBh

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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mt TEFM

Plasmid

#205058

Purpose

TEFM protein expression

Depositor

Smita Patel

Article

Sultana et al J Biol Chem. 2017 Nov 3;292(44):18145-18

Insert

TEFM (TEFM Human)

Expression

Bacterial

Mutation

Codon optimized

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBEL2511

Plasmid

#195625

Purpose

Complementation of MlaA(WT)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAC155-pCR8-sgExpression

Plasmid

#49045

Purpose

U6-driven sgRNA expressing cassette on a gateway donor vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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Phi3-iRFP

Plasmid

#106296

Purpose

CMV driven iRFP with n terminal membrane binding domain of GAP43

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

iRFPmem

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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BSP605

Plasmid

#122250

Purpose

expression vector for mCerulean under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mCerulean

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJCC_083 Cas12i1

Plasmid

#171670

Purpose

For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

Cas12i1

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSBDEST.Z

Plasmid

#79470

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Zeocin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-Rabin8

Plasmid

#24910

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

Rabin8 (RAB3IP Human)

Use

Gateway Cloning

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

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CMV566 A-USF

Plasmid

#33360

Depositor

Charles Vinson

Article

Qyang et al Mol Cell Biol. 1999 Feb;19(2):1508-17.

Insert

USF (Usf1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant Negative (see notes below)

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only
pucKan_ARS_pgkZeo_ncx1_mCherry_2TA_Puro_ARS

Plasmid

#163755

Purpose

piggybac transposon encoding human Ncx1 gene promoter-driven mCherry and puromycin-acetyltransferase for labelling and selection of pluripotent stem cell-derived cardiomyocytes

Depositor

Kurt Pfannkuche

Article

Hamad et al Methods Mol Biol. 2021 Oct 19. doi: 10.1

Insert

mCherry

Use

Piccybac transposon

Tags

puromycin-N-acetyltransferase

Expression

Mammalian

Promoter

human NCX1

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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PE_26: Para_Lac_Tet_eG::sfYFP

Plasmid

#104887

Purpose

PE_26: Para_Lac_Tet_eG::sfYFP

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter Para_Lac_Tet_eG

Expression

Bacterial

Promoter

Para_Lac_Tet_eG

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO C1-APEX2

Plasmid

#129641

Purpose

Express ER-localized C1-APEX2 fusion in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

C1(1-29)-APEX2-GFP

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T2_AmpR

Plasmid

#119159

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T3_AmpR

Plasmid

#119160

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T1_AmpR

Plasmid

#119158

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pWH-DI

Plasmid

#47385

Purpose

Expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Inserts

dxs
idi

Expression

Bacterial

Promoter

PxylA

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only
LV-TRE-VP16 mouse MyoD-T2A-dsRedExpress2

Plasmid

#60626

Purpose

Expresses VP16 mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP16 mouse MyoD

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMS17: pTwinStrep-SUMO-AvTnsD

Plasmid

#168150

Purpose

AvTnsD expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsD

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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