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pAAV-EF1a-fDIO-stGtACR2-FusionRed

Plasmid

#217975

Purpose

AAV transfer plasmid to express stGtACR2-FusionRed in a frt-dependent manner

Depositor

Edward Callaway

Article

Kirchgessner et al Curr Biol. 2021 Dec 6;31(23):5121-5137.e

Insert

stGtACR2-FusionRed

Use

AAV

Tags

FusionRed

Promoter

EF1a

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFRTpsiCHECK_SEAP

Plasmid

#154461

Purpose

Expression of SEAP and firefly luciferase

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

SEAP

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-mTagBFP2-2A

Plasmid

#102602

Purpose

Control lentivector. Coexpression of mTagBFP and gene of interest from CMV promoter

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHMGCS-Luc (aka: pES8)

Plasmid

#14947

Depositor

Axel Nohturfft

Article

Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

HMG CoA synthase promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

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pDA007

Plasmid

#131380

Purpose

ORFeus expression, WT

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus-Hs

Tags

ORF2-3xFlag

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT001

Plasmid

#182711

Purpose

aTc inducible dCasRx

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

dCasRx

Use

CRISPR

Tags

2x-FLAG

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pTet

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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PX459-APP-KO

Plasmid

#176487

Purpose

To knockout APP gene

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

gRNA sequence

Use

CRISPR

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR221-mCherry

Plasmid

#79509

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

mCherry RFP

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-TMCC1

Plasmid

#120932

Purpose

Expresses human TMCC1 in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Insert

Trans membrane and coiled coil domain containing protein 1 (TMCC1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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SB52

Plasmid

#21487

Depositor

Shoumo Bhattacharya

Article

Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

Insert

Cited2 (CITED2 Human)

Expression

Mammalian

Available Since

July 23, 2009

Availability

Academic Institutions and Nonprofits only
ss-SigmaR1DTM-mNeonGreen

Plasmid

#226575

Purpose

Encodes mutant SigmaR1 protein (transmembrane region deletion) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Smad2-3'UTR

Plasmid

#61775

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pBEL1092

Plasmid

#195635

Purpose

Expression/purification of 6xHis-TEV-sfGFP

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Smad5-3'UTR

Plasmid

#61777

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad5

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad5 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
mRuby2-Rab5a-7

Plasmid

#55911

Purpose

Localization: Ras GTPase, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Rab5a

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAC103-PBNeo-U6-sgRNA-Expression

Plasmid

#48228

Purpose

sgRNA expression on PiggyBac vector with Neo-selectable marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM60-Ptb

Plasmid

#38245

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Ptb (Ptbp1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pLck-mVenus-C1

Plasmid

#84337

Purpose

Expresses a mVenus tagged with a membrane localization sequence

Depositor

Dorus Gadella

Article

van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

Insert

Lck (MGCVCSSNPELPVAT)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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p{CFD4-3xP3::DsRed}

Plasmid

#86864

Purpose

Expresses DsRed in eyes and provides a backbone for expressing two gRNAs. With attB integration site.

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Type

Empty backbone

Expression

Insect

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pBI121-GFP-RmMYB108

Plasmid

#173181

Purpose

Expresses Rosa multiflora MYB108 for subcellular localization in plants

Depositor

Daidi Che

Article

Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

Insert

RmMYB108

Expression

Plant

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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