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JH204: pMVP (R4-R3) human PDX1 open

Plasmid

#121745

Purpose

pMVP R4-R3 entry plasmid, contains human PDX1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Human PDX1 (open) (PDX1 Human)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B G120A no-stop

Plasmid

#123206

Purpose

Gateway entry clone encoding human MAP1LC3B G120A lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway Cloning

Mutation

Glycine 120 to Alanine, stop codon removed

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL1 no-stop

Plasmid

#123210

Purpose

Gateway entry clone encoding human GABARAPL1 lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Gateway Cloning

Mutation

Stop codon removed

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL2 no-stop

Plasmid

#123211

Purpose

Gateway entry clone encoding human GABARAPL2 lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Gateway Cloning

Mutation

Stop codon removed

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-2B-Nkx2-5 Y191C

Plasmid

#98613

Purpose

Gateway entry vector for NKX2-5 Y191C

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 Y191C (Nkx2-5 Mouse)

Use

Gateway Cloning

Mutation

Point mutation Y191C

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR CMVie-Lifeact-EGFP L1-R5

Plasmid

#84391

Purpose

Multisite Gateway entry vector for expression of EGFP-tagged Lifeact in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Gateway Cloning

Tags

EGFP

Expression

Mammalian

Promoter

CMV immediate early

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1B07

Plasmid

#70819

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CkIIalpha (CkIIalpha Fly)

Use

Gateway Cloning

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL4-no stop

Plasmid

#67323

Purpose

hArl4 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 105 A to T; silent mutation

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL5A

Plasmid

#67324

Purpose

hArl5a with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 70 C to T; silent mutation

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL5A-no stop

Plasmid

#67325

Purpose

hArl5a without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 70 C to T; silent mutation

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL6

Plasmid

#67328

Purpose

hArl6 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL4C-no stop

Plasmid

#67331

Purpose

hArl4c without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL4D

Plasmid

#67336

Purpose

hArl4D with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL14

Plasmid

#67338

Purpose

hArl14 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 172 C to T; aa L58F

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL14-no stop

Plasmid

#67339

Purpose

hArl14 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 172 C to T; aa L58F

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARFRP1

Plasmid

#67342

Purpose

hArfrp1 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARFRP1 (ARFRP1 Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARF1-no stop

Plasmid

#67307

Purpose

hArf1 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARF3-no stop

Plasmid

#67309

Purpose

hArf3 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF3 (ARF3 Human)

Use

Gateway Cloning

Expression

Bacterial

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARF4-no stop

Plasmid

#67311

Purpose

hArf4 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

V53A, L123P and M134I mutations were unintended P…

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARF6-no stop

Plasmid

#67315

Purpose

hArf6 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only