We narrowed to 7,705 results for: ALP

1. pEBB COMMD6 Flag

   Plasmid

   #74898

   Purpose

   Express COMMD6 in mammalian cells

   Depositor

   Colin Duckett, Ezra Burstein

   Article

   Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

   Insert

   COMMD6 (COMMD6 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF-1alpha promoter

   Available Since

   June 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMpGWB321

   Plasmid

   #68649

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   Modified EAR motif plant-specific repression doma…

   Expression

   Plant

   Promoter

   MpEF1alpha promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HSF1 KO

   Plasmid

   #200207

   Purpose

   gRNA for HSF1 KO

   Depositor

   Sheila Teves

   Article

   Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

   Insert

   HSF1 KO (Hsf1 Mouse)
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBS NL4-3 envFS eGFP 5'CMV Δtat

   Plasmid

   #101347

   Purpose

   tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Mutation

   delta Tat

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFETCh_GATAD2A

   Plasmid

   #86279

   Purpose

   Donor vector for 3' FLAG tag of human GATAD2A

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   GATAD2A homology arms (GATAD2A Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBS-AMV-α1L9'S-nAchR

   Plasmid

   #20684

   Depositor

   Henry Lester

   Article

   Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

   Insert

   nicotinic acetylcholine receptors (Chrna1 Mouse)
   

   Tags

   AMV and pA 50

   Expression

   Bacterial

   Mutation

   Leucine at 9' position in M2 region changed …

   Available Since

   April 3, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pCellFree_G03 GTF2E1

   Plasmid

   #67089

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GTF2E1 (GTF2E1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pSicoR-Ef1a-mCh-GFPi

   Plasmid

   #31849

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   GFP RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   U6 for shRNA; Ef1alpha for mCherry

   Available Since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLJM60-S6K1(T389S) barcoded

   Plasmid

   #48800

   Purpose

   expresses mutant S6K1

   Depositor

   David Sabatini

   Article

   Kang et al Science. 2013 Jul 26;341(6144):1236566.

   Insert

   S6K1 T389S (Rps6kb1 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG and barcode: GGTACC

   Expression

   Mammalian

   Mutation

   T389S

   Promoter

   CMV

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-sCAG-GluClβ.CreON

    Plasmid

    #196996

    Purpose

    Cre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.

    Depositor

    David Bennett

    Article

    Middleton et al Pain. 2023 Jun 27. doi: 10.1097/j.pain.0

    Insert

    GluClβ -YFP
    

    Use

    AAV and Cre/Lox

    Tags

    YFP

    Promoter

    short CAG

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMpGWB121

    Plasmid

    #68575

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Promoter

    MpEF1alpha promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. miniSOG-Tubulin-6

    Plasmid

    #57777

    Purpose

    Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Tubulin (TUBA1B Human)
    

    Tags

    miniSOG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pSicoR-Ef1a-mCh-Puro-Luci

    Plasmid

    #31850

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    Luciferase RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available Since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pONSY-mCherry

    Plasmid

    #111874

    Purpose

    This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJT112_GalL_nCDA1-xBE3

    Plasmid

    #141318

    Purpose

    Expressing base editor nCDA1-xBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-xBE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

    Promoter

    GalL

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF1α-squirrel-monkey-CHMP3(155)-HA

    Plasmid

    #154173

    Purpose

    expresses squirrel monkey CHMP3(155) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3(155)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal truncation of CHMP3 at amino acid 155

    Promoter

    EF1-alpha

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX458_CEBPA_1

    Plasmid

    #86358

    Purpose

    Encodes gRNA for 3' target of human CEBPA

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against CEBPA (CEBPA Human)
    

    Use

    CRISPR

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pProEX-Af2Sir2

    Plasmid

    #8380

    Depositor

    Cynthia Wolberger

    Article

    Avalos et al Mol Cell 2002 Sep;10(3):523-35.

    Insert

    Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

19. mRuby2-Tubulin-6

    Plasmid

    #55914

    Purpose

    Localization: Microtubules, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Tubulin (TUBA1B Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pEF1α-squirrel-monkey-retroCHMP3-T2A-H2B-BFP

    Plasmid

    #154185

    Purpose

    expresses squirrel monkey retroCHMP3 with self-cleaving tag in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    retroCHMP3
    

    Tags

    HA and T2A-H2B-BFP

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge