We narrowed to 42,187 results for: ids

1. pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP

   Plasmid

   #35499

   Purpose

   AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Has Service

   AAV9

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-DIO C1V1 (t/t)-TS-EYFP

   Plasmid

   #35497

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Has Service

   AAV9

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   Ef1a

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry

   Plasmid

   #35504

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   hChR2(C128S/D156A)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C128S and D156A

   Promoter

   Ef1a

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHis-LMW_FGF2

   Plasmid

   #157657

   Purpose

   Expresses FGF2 in E.coli cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Fibroblast growth factor 2 (FGF2 Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changed Cystein 211,229 to serine, deleted amino …

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTwist-WIV1-CoV Δ18

   Plasmid

   #164439

   Purpose

   Encodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   WIV1-CoV Spike truncated to remove 18 amino acids
   

   Expression

   Mammalian

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRES-hrGFP II-mTET1_CD

   Plasmid

   #83571

   Purpose

   Expresses a mutant catalytic domain of TET1 in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Mutation

   TET1 amino acids 1-1417 deleted, catalytic domain…

   Promoter

   CMV

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA

   Plasmid

   #211817

   Purpose

   AAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNA

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   N-terminus of SpCas9-ABE8e and tracrRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   chicken β-actin promoter and U6 promoter

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry

   Plasmid

   #35502

   Purpose

   AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   hChR2(C128S/D156A)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C128S and D156A

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. KAT6B-luciferase

   Plasmid

   #113353

   Purpose

   evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   KAT6B-enhancer (KAT6B Human)
   

   Use

   Luciferase

   Available Since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAcBac1-Ma-sfGFP WT

    Plasmid

    #197567

    Purpose

    Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP WT
    M. alvus Pyl-tRNA (6) (4x copies)
    

    Tags

    V5-His6

    Expression

    Mammalian

    Promoter

    CMV and U6/H1

    Available Since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSUMO CPTP

    Plasmid

    #170735

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    CPTP (CPTP Human)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-Drosha1-390

    Plasmid

    #62521

    Purpose

    C-terminal aa391-1374 of Drosha is deleted

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    human Drosha with aa 391-1374 deleted (DROSHA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 391-1374

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)

    Plasmid

    #120296

    Purpose

    AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    Rosa26 gRNA (SpCas9 scaffold)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. HOXB3 (human) FLAG pSP65

    Plasmid

    #8523

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB3 (HOXB3 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Contains 5 additional amino acids before ATG from…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

15. GFP-Drosha1-850

    Plasmid

    #62522

    Purpose

    C-terminal aa851-1374 of Drosha is deleted

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    human Drosha with aa 851-1374 deleted (DROSHA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 851-1374

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pEGFP-RNF8-Middle-part

    Plasmid

    #64672

    Purpose

    Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    ring finger protein 8 (RNF8 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Containing only middle part of RNF8 amino acids 1…

    Promoter

    CMV

    Available Since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pFLAG R106L-CPTP

    Plasmid

    #170744

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    CPTP (CPTP Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R106L

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFLAG K60A-CPTP

    Plasmid

    #170743

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    CPTP (CPTP Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K60A

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p-AAV2-hSyn-MerMAID6-Citrine

    Plasmid

    #126521

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID6
    

    Use

    AAV

    Tags

    Citrine

    Promoter

    human synapsin

    Available Since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

    Plasmid

    #120294

    Purpose

    AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    C-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    split-intein

    Expression

    Mammalian

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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