We narrowed to 10,418 results for: 158

1. pJT300 Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast

   Plasmid

   #195542

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI

   Plasmid

   #126480

   Purpose

   Expresses Cas9-GSAlinker-TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   Cas9
   GSA linker
   TdT (DNTT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed codons to reduce repetitive sequences.

   Promoter

   CMV

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_FH_WT

   Plasmid

   #82210

   Purpose

   Gateway Donor vector containing FH , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   FH (FH Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pF1-Hs Cdk1/cyclin B1

   Plasmid

   #177330

   Purpose

   Co-expression of the human active Cdk1/cyclin B1 kinase complex

   Depositor

   Andreas Boland

   Article

   Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

   Inserts

   Cyclin-dependent kinase 1 (CDK1 Human)
   G2/mitotic-specific cyclin-B1 (CCNB1 Human)
   

   Use

   Tags

   2 x Strep-tag and 8 x His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEos3.2GG

   Plasmid

   #203756

   Purpose

   Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   KRas c-term (KRAS Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMV4 lenti pEF-rTetR(SE-G72P)-3XFLAG-ANM2(SH3_1)-T2A-mCherry-BSD-WPRE

   Plasmid

   #188750

   Purpose

   Lentiviral backbone containing ANM2 SH3_1 domain as a fusion with rTetR(SE-G72P)-3XFLAG

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   ANM2 SH3_1 (PRMT2 Human)
   

   Use

   Lentiviral

   Tags

   rTetR(SE-G72P)-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

   Plasmid

   #217808

   Purpose

   Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   T7

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiGuide-puro-NLS-GFP

   Plasmid

   #185473

   Purpose

   Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDONR223_BTK_WT

   Plasmid

   #82197

   Purpose

   Gateway Donor vector containing BTK , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   BTK (BTK Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiCRISPRv2_RB1#2

    Plasmid

    #174151

    Purpose

    Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    RB1 (RB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_IRF4_WT

    Plasmid

    #81782

    Purpose

    Gateway Donor vector containing IRF4 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    IRF4 (IRF4 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-Kif2c

    Plasmid

    #208651

    Purpose

    Mammalian expression plasmid expressing eGFP-Kif2c driven by CMV promoter

    Depositor

    Samuel Bakhoum

    Article

    Li et al Nature. 2023 Aug;620(7976):1080-1088. do

    Insert

    Kif2c (KIF2C Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA4-AMBRA1-3xFLAG

    Plasmid

    #174157

    Purpose

    Vector for expression of 3xFLAG-tagged wild-type AMBRA1

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pD649-HAsp-KIR3DL2-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156758

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR3DL2 (KIR3DL2 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_XPO1_WT

    Plasmid

    #81812

    Purpose

    Gateway Donor vector containing XPO1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    XPO1 (XPO1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-hCCND1-T2A-hCCND2-P2A-hCCND3

    Plasmid

    #174156

    Purpose

    Lentiviral vector expressing all three D-type cyclins (cyclin D1, cyclin D2, cyclin D3) from a single promoter

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    CCND1>T2A>CCND2>P2A>CCND3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. His-GST-TEV-BRD4(42-459) BD1-BD2

    Plasmid

    #233887

    Purpose

    pGex for protein overexpression of human BRD4

    Depositor

    Alessio Ciulli

    Article

    Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    His-GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223_PER1_WT

    Plasmid

    #82152

    Purpose

    Gateway Donor vector containing PER1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PER1 (PER1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-DIO-EF1α-mKate2-PDE4D3-Cat

    Plasmid

    #169128

    Purpose

    AAV plasmid containing a mKate2 and a truncated PDE4D3

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Has Service

    AAV1

    Insert

    mKate2-PDE4DCatL (PDE4D Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    truncated, Catalatic domain only

    Promoter

    EF1a

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiCRISPRv2_AMBRA1#2

    Plasmid

    #174153

    Purpose

    Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA (AMBRA1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron

    Plasmid

    #235087

    Purpose

    GFP splicing reporter with FRG1 intron

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron (FRG1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    custom bidirectional

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBoBi-hGOLGA2-C-PEN2

    Plasmid

    #183649

    Purpose

    Lentiviral expression of a golgi-residential PEN2.

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    PEN2 and GOLGA2 (PSENEN, GOLGA2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    WT

    Promoter

    CMV

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

    Plasmid

    #154430

    Purpose

    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

    Depositor

    David Liu

    Article

    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

    Insert

    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    VRQR point mutations in SpCas9

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDONR223_SETDB1_WT

    Plasmid

    #82291

    Purpose

    Gateway Donor vector containing SETDB1, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SETDB1 (SETDB1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR223_STAT3_WT

    Plasmid

    #82235

    Purpose

    Gateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDONR223_BRAF_p.V600E

    Plasmid

    #81700

    Purpose

    Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    BRAF (BRAF Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    V600E

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDNA2.0 6H-FLAG-TEV-KRAS G12C

    Plasmid

    #111848

    Purpose

    Expresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

    Insert

    KRAS G12C (KRAS Human)
    

    Use

    Tags

    6xHis-tag, FLAG-tag, and TEV protease cleavage se…

    Expression

    Bacterial

    Mutation

    Changed Glycine 12 to Cysteine

    Promoter

    T5

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR223_IRS1_WT

    Plasmid

    #82214

    Purpose

    Gateway Donor vector containing IRS1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    IRS1 (IRS1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-SpyTag-Flag-SRSF1-V5-mCherry

    Plasmid

    #235089

    Purpose

    SRSF1 mCherry fusion protein without MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-SpyTag-Flag-SRSF1-V5-mCherry (SRSF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVX-KIF2C

    Plasmid

    #205996

    Purpose

    inducible expression of HA-KIF2C

    Depositor

    Floris Foijer

    Article

    Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

    Insert

    KIF2c (KIF2C Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    pTight

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge