We narrowed to 40,729 results for: kan

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P-Ef1a-RPL22-3XFlag-P2A-mCherry-T2A-Puro

Plasmid

#170322

Purpose

Expresses FLAG and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR S286/287/291A

Plasmid

#159160

Purpose

Expresses DEPTOR S286/287/291A mutant in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Mutation

changed serine 286,287 and 291 to alanine

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR S286/287/291D

Plasmid

#159161

Purpose

Expresses DEPTOR S286/287/291D mutant in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Mutation

changed serine 286,287 and 291 to aspartic acid

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6_YAP1-V5

Plasmid

#173002

Purpose

constitutive expression of V5-tagged YAP1

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

YAP1-V5 (YAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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SGyA

Plasmid

#160292

Purpose

Drives expression of Cas9 and a TdTomato fluorescent marker

Depositor

Omar Akbari

Article

Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

Inserts

Vas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome

Tags

T2A GFP

Expression

Insect

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSD138A

Plasmid

#218130

Purpose

Hybrid circuit displaying hsCRBN bait (P22 anchor) and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN (CRBN Human)
luxAB

Tags

scP22

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS

Plasmid

#145268

Purpose

Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(+10A)-IDR (RUNX2 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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retro-puro vector

Plasmid

#58250

Purpose

Retroviral expression vector encoding an empty IRES-Puromycin cassette

Depositor

Georgios Stathopoulos

Article

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 E17K

Plasmid

#73050

Purpose

Human AKT3 E17K

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 E17K (AKT3 Human)

Expression

Mammalian

Mutation

E17K

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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SHMT2-RES

Plasmid

#72880

Purpose

overexpression

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

SHMT2 (SHMT2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

silent mutations in site corresponding to shSHMT2…

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3/hArf5(WT)-mCherry

Plasmid

#79421

Purpose

Expresses C-terminally mCherry-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf5 (ARF5 Human)

Tags

mCherry

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP

Plasmid

#170327

Purpose

Double-floxed inverse Orientation HA and IRES-YFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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Chr1q_Cassette-Integration_gRNA

Plasmid

#195126

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG DEPTOR (13×S/T->A)

Plasmid

#21702

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

DEPTOR 13xA (DEPTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

Nonphosphorylatable mutant. 13xA. Also has N204S …

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[1,2]-N (TRP1)

Plasmid

#177797

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[1,2]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCX0393

Plasmid

#205984

Purpose

Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Inserts

Ga0116245_10002768 candidate type VII proteins
Cas14
Cas5
truncated CRISPR array

Tags

14xHis and TwinStrep-tag

Expression

Bacterial

Promoter

T7 (proteins), pJ23119 (crRNA)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pJM185A

Plasmid

#218126

Purpose

Hybrid circuit displaying hsCRBN bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN (CRBN Human)
luxAB

Tags

434cI_RR69

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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Dom neg CstF64

Plasmid

#127250

Purpose

Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

CstF64 delta 282 (CSTF2 Human)

Use

Other

Expression

Mammalian

Mutation

insert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…

Promoter

pEF1a

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-PFDN2

Plasmid

#202664

Purpose

Tet-ON 3rd generation expression vector with inducible expression of PFDN2 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

prefoldin subunit 2 (PFDN2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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TKTL1

Plasmid

#72419

Purpose

Study the role of aberrant expression of TKTL1 in HNSCC tumorigenesis

Depositor

David Sidransky

Article

Sun et al Clin Cancer Res. 2010 Feb 1;16(3):857-66

Insert

TKTL1 (TKTL1 Human)

Expression

Mammalian

Mutation

may have one or two synonymous substitutions

Promoter

CMV

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only