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pInducer-YAP1-S127A/S397A

Plasmid

#213585

Purpose

Doxycycline inducible expression of YAP1-S127A/S397A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

S127A, S397A

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-YAP1-S127A/S397A/S94A

Plasmid

#213586

Purpose

Doxycycline inducible expression of YAP1-S127A/S397A/S94A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

S127A, S397A, S94A

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-BSD

Plasmid

#224122

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-BSD

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4-AARE-luc2P-Hygro

Plasmid

#101787

Purpose

Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

3xAARE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

minimal TATA-box promoter with low basal activity

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-P2A-BSD

Plasmid

#174819

Purpose

Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PE2-P2A-BSD

Tags

P2A-BSD and SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDule2-3-nitroTyrosine (A7)

Plasmid

#174079

Purpose

Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

Insert

3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Expression

Bacterial

Mutation

Y32H H70T D158H I159A L162R

Promoter

GlnRS (constitutive)

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_CD63-SBP-EGFP

Plasmid

#247324

Purpose

Synchronize the trafficking of CD63 from the ER.

Depositor

Franck Perez, Clotilde Thery

Article

Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

Insert

CD63 (CD63 Human)

Tags

SBP-EGFP (inserted in the small luminal loop of C…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_CD63YA-SBP-EGFP

Plasmid

#222327

Purpose

Synchronize the trafficking of CD63YA from the ER.

Depositor

Franck Perez

Article

Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

Insert

Streptavidin-KDEL and CD63-Y235A fused to SBP-EGFP (CD63 Human)

Expression

Mammalian

Mutation

Tyr235 to Ala

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn CaBLAM

Plasmid

#244227

Purpose

Bioluminescent reporter for calcium signaling in neurons

Depositor

Ute Hochgeschwender

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Inserts

mNeonGreen
CaBLAM

Use

AAV

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP6

Plasmid

#141260

Purpose

Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-B2M

Plasmid

#116715

Purpose

Lentiviral expression of B2M

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

B2M (B2M Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-VDAC1 [N152B/23R]

Plasmid

#177502

Purpose

Mammalian Expression Plasmid of anti-VDAC1 (Human). Derived from hybridoma N152B/23.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VDAC1 (Homo sapiens) recombinant mouse monoclonal antibody (VDAC1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP-proα2(I)-G610C

Plasmid

#119827

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-KGRQtoSYAA-shRNAres_W

Plasmid

#147901

Purpose

Mammalian Expression of HsNot1iso1-KGRQtoSYAA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-KGRQtoSYAA-shRNAres (CNOT1 Human)

Tags

GFP (G4C mutation)

Expression

Mammalian

Mutation

NM_016284.4, K1426S, G1451Y, R1458A, Q1549A

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CD

Plasmid

#116773

Purpose

Lentiviral expression of PIK3CD

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CD (PIK3CD Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Mb-sfGFP 150TAG

Plasmid

#197570

Purpose

Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP 150TAG
Pyl-tRNA (4 copies)

Tags

V5-His6

Expression

Mammalian

Mutation

N150TAG

Promoter

CMV and U6/H1

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pFUSE2ss-CLIg-mK_M18

Plasmid

#82358

Purpose

Expression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

Insert

mouse immunoglobulin kappa light chain, variable fragment derived from clone M18 (Igk, Igkc, Igk-V Mouse)

Tags

none

Expression

Mammalian

Promoter

hEF1-HTLV prom

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV40-EF1a-hBCL10-SO

Plasmid

#75256

Purpose

retroviral expression vector for expression of StrepOne-Tagged human BCL10

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

Bcl10 (BCL10 Human)

Use

Retroviral

Tags

StrepOne Tag

Expression

Mammalian

Promoter

pMSCV-LTRs, EF1a

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-NR4A1-P2A-mCherry

Plasmid

#203859

Purpose

Tet-inducible lentiviral plasmid expressing NR4A1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

NR4A1 (NR4A1 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-ZEB2-P2A-mCherry

Plasmid

#203857

Purpose

Tet-inducible lentiviral plasmid expressing ZEB2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

ZEB2 (ZEB2 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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