We narrowed to 10,238 results for: ada

1. p-AAV2-hSyn-MerMAID6-Citrine

   Plasmid

   #126521

   Purpose

   Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

   Insert

   MerMAID6
   

   Use

   AAV

   Tags

   Citrine

   Promoter

   human synapsin

   Available Since

   Nov. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. ApoE2_mCh-SspB (pBS1142)

   Plasmid

   #185324

   Purpose

   For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE2
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKCAG-H2BmCherry-OptoJNKi

   Plasmid

   #89741

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi
   

   Use

   Optogenetics

   Tags

   Histone2B and mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBullet-cg-c

   Plasmid

   #53070

   Purpose

   destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

   Depositor

   Joshua Heazlewood

   Article

   Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. deltaVP1 + VP2C-GFP

   Plasmid

   #166676

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-GFP
   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDN-G1GZmbh

   Plasmid

   #44516

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
   yEGFP::ZeoR
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Expression

   Yeast

   Mutation

   Two tandem tet operators (2XtetO2) downstream of …

   Promoter

   PGal1-D12 promoter

   Available Since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_SEPT2 NCmut-mApple

   Plasmid

   #180321

   Purpose

   mammalian expression of human SEPT2 NCmut fused to monomeric Apple

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN2 (SEPTIN2 Human)
   

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   SEPT2 F20D, V27D

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_SEPT9_i1-i5-msfGFP

   Plasmid

   #180332

   Purpose

   mammalian expression of human SEPT9_i1-i5 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v7 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1-i5

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBullet-vac-c

   Plasmid

   #53076

   Purpose

   destination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

   Depositor

   Joshua Heazlewood

   Article

   Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBullet-er-c

    Plasmid

    #53068

    Purpose

    destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ECFP and EYFP

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_UdgX-EE-UdgX-NG-nCas9

    Plasmid

    #163570

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-ABEMAX-NLS-P2A_BLAST

    Plasmid

    #135355

    Purpose

    Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    ABEMax-2XNLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pfast bac cry1myc

    Plasmid

    #51892

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    cry1 (Cry1 Mouse)
    

    Tags

    3x Flag and myc

    Promoter

    polyhedrin promoter

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pBullet-cyt-c

    Plasmid

    #53066

    Purpose

    destination vector with cytosol marker (ECFP) for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ECFP and EYFP

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pfast bac Rbx-HA

    Plasmid

    #51893

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    Rbx1 (RBX1 Human)
    

    Tags

    HA

    Promoter

    polyhedrin promoter

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCI-Neo-gp78 Cue-m1,2 / JM22

    Plasmid

    #13305

    Depositor

    Allan Weissman

    Article

    Chen et al Proc Natl Acad Sci U S A. 2006 Jan 10. 1

    Insert

    gp78 (AMFR Human)
    

    Expression

    Mammalian

    Mutation

    M467G ; F468G ; P469R ; V476R ; D479V ; L480D

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5-FRT-T0-eGFP-HROB

    Plasmid

    #135299

    Purpose

    Expresses eGFP-tagged HROB in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Hustedt et al Genes Dev. 2019 Oct 1;33(19-20):1397-141

    Insert

    HROB (HROB Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCSIIneo-TRE-hPhyB621- mCherry-HRasCT

    Plasmid

    #139482

    Purpose

    Expresses PhyB621-mCherry-HRasCT in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    PhyB621 (PHYB Mustard Weed)
    

    Use

    Lentiviral

    Tags

    mCherry-HRasCT

    Expression

    Mammalian

    Mutation

    G229L and deletion of aa Y at position 235 in mch…

    Promoter

    Tet responsible element

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBullet-cg-n

    Plasmid

    #53069

    Purpose

    destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (N-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ERL1 C11_pECIA14

    Plasmid

    #114929

    Purpose

    Prey vector ERL1 C11_pECIA14 should be used with bait vector ERL1 C11_pECIA2.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT5G62230
    

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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