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pJBL2191

Plasmid

#71205

Purpose

pbuE STAR Antisense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

pbuE STAR Antisense

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23119

Availability

Academic Institutions and Nonprofits only

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pJBL1864

Plasmid

#71210

Purpose

Anti-anti Sense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

Anti-anti Sense

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23119

Availability

Academic Institutions and Nonprofits only

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pLVX-PERK(LD)-EGFP-HOTag3

Plasmid

#225678

Purpose

Used to generate stable cell lines that express PERK(LD)-EGFP-HOTag3 and to detect the unfolded proteins in the ER lumen.

Depositor

Likun Wang

Article

Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET_RTX_(exo-)

Plasmid

#102786

Purpose

Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

Depositor

Andrew Ellington

Article

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase with 3'-5' deficient exonuclease

Expression

Bacterial

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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Venus Cam KII alpha

Plasmid

#29428

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CAM KII Alpha

Tags

Venus

Expression

Mammalian

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pPSU20-40-60-80

Plasmid

#173839

Purpose

Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACCBPv3

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-SEpHluorin

Plasmid

#32001

Depositor

Sergio Grinstein

Article

Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

Insert

mCherry

Tags

Superecliptic pHluorin

Expression

Mammalian

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pIYC08

Plasmid

#64743

Purpose

Yeast expression plasmid: acsL641P, ALD6, ADH2, ERG10

Depositor

Jens Nielsen

Article

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Insert

ADH2, ALD6, acsL641P, ERG10

Expression

Yeast

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pQacR-Q1

Plasmid

#74689

Purpose

QacR-Q1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSQ1-QacR-PQacR-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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GST-RH▲-MNase

Plasmid

#136292

Purpose

Expression of catalytic inactive RNase H fused to micrococcal nuclease

Depositor

Kavitha Sarma

Article

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

RNase HI-D10R-E48R and MNase

Expression

Bacterial

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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SUMO_TwinStrep_Cas7-11_crRNA

Plasmid

#197612

Purpose

For protein purification of Cas7-11

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

SUMO_TwinStrep_Cas7-11 with crRNA

Expression

Bacterial

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_PsCatCh2.0-YFP

Plasmid

#186978

Purpose

Expresses PsCatCh2.0 channelrhodopsin in mammalian cells

Depositor

Adam Cohen

Article

Hayward et al eLife. 2023 Oct 6;12:RP86833. doi: 10.75

Insert

PsCatCh2.0-YFP

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

Plasmid

#138315

Purpose

Lenti expression of mTgBFP2

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

mTAGBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-RapGAP

Plasmid

#118324

Purpose

Expression of RapGAP

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

RapGAP

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTAKN2_J23119-sgRNA

Plasmid

#108552

Purpose

Bacterial sgRNA expression vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

2x Streptococcus pyogenes sgRNA

Expression

Bacterial

Promoter

J23119

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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PB-U6

Plasmid

#120358

Purpose

piggyBac transposon vector for sgRNA expression.

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPSU15

Plasmid

#173828

Purpose

Expresses 15 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

HSTPABPAC

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET_RTX

Plasmid

#102787

Purpose

Expresses the Reverse Transcriptase gene

Depositor

Andrew Ellington

Article

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase

Expression

Bacterial

Promoter

T7 promoter + lac operator

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EGFP-HOTag3

Plasmid

#225677

Purpose

Used to generate stable cell lines that express EGFP-HOTag3. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

Depositor

Likun Wang

Article

Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-sfCherry(1-10)

Plasmid

#70222

Purpose

Expresses sfCherry(1-10) in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry(1-10)

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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