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pIII/MS2-1

Plasmid

#220629

Purpose

Yeast/E. coli shuttle vector in which an RNA polymerase III promoter directs transcription of an RNA containing two tandem MS2 sites.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pIII/MS2-2

Plasmid

#220630

Purpose

For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+/ER-2mutAEQ

Plasmid

#45541

Depositor

Javier Alvarez-Martin

Article

de la Fuente et al Biochem J. 2012 Aug 1;445(3):371-6. doi:

Insert

ER-2mutAEQ

Tags

ER targeted

Expression

Mammalian

Mutation

Asp119Ala and Asn28Leu

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23100-RiboJ-UTR1-E0040m-B0015)

Plasmid

#197662

Purpose

Expresses GFP under the control of J23100 and RiboJ-UTR1

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23106-RiboJ-UTR1-E0040m-B0015)

Plasmid

#197674

Purpose

Expresses GFP under the control of J23106 and RiboJ-UTR1

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23100-RiboJ-BCD2-E0040m-B0015)

Plasmid

#197661

Purpose

Expresses GFP under the control of J23100 and RiboJ-BCD2

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23103-RiboJ-BCD2-E0040m-B0015)

Plasmid

#197667

Purpose

Expresses GFP under the control of J23103 and RiboJ-BCD2

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMK-N. tabacum atpB/accD

Plasmid

#123582

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

N. tabacum atpB/accD integration site

Use

Chloroplast transformation vector

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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XE244 Mouse Naked-1 CS2p+

Plasmid

#16728

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Mouse Naked-1 (Nkd1 Mouse)

Use

Xenopus expression

Tags

His and myc

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
BS-Ura3-GFP-Atg8

Plasmid

#69194

Purpose

Single copy integration of GFP-Atg8, use auxotrophic marker URA3(Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

GFP-Atg8

Tags

EGFP

Expression

Yeast

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pMK-N. tabacum trnI/trnA

Plasmid

#123579

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

N. tabacum trnI/trnA integration site

Use

Chloroplast transformation vector

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLRD14

Plasmid

#208266

Purpose

Production of syn-pimara-7,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL4

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD17

Plasmid

#208269

Purpose

Production of syn-stemod-13(17)-ene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL11

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP-ATG13(315)

Plasmid

#122072

Purpose

Expressing GFP-Atg13

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

GFP

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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GP757

Plasmid

#164540

Purpose

Expresses Sstr3-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Somatostatin receptor type 3 (Sstr3 Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

WT

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-mPlexinA2

Plasmid

#16423

Depositor

Jonathan Raper

Article

Brown et al Development. 2001 Aug . 128(16):3071-80.

Insert

Plexin A2 (Plxna2 Mouse)

Use

In situ template

Mutation

None

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pET28a-Hisx6-Sirt6-H133Y

Plasmid

#20279

Depositor

Leonard Guarente

Article

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Tags

Hisx6

Expression

Bacterial

Mutation

Histidine 133 to Tyrosine

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
PHF20 (3QII)

Plasmid

#32039

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

PHF20 (PHF20 Human)

Tags

His (with TEV cleavage)

Expression

Bacterial

Mutation

Tudor domain 2 only (amino acids 83-150)

Promoter

T7

Available Since

Aug. 8, 2012

Availability

Industry, Academic Institutions, and Nonprofits
XE245 Mouse Naked-2 CS2p+

Plasmid

#16727

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Mouse Naked-2 pCS2P+F (Nkd2 Mouse)

Use

Xenopus expression

Mutation

*

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-Hisx6-Sirt6-S56A

Plasmid

#20278

Depositor

Leonard Guarente

Article

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Tags

Hisx6

Expression

Bacterial

Mutation

Serine 56 to Alanine

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only