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Showing: 1241 - 1280 of 18068 results

  14. pREDCas9

    Plasmid
    #71541
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    lambda red genes
    plasmid curing system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71541-plasmid-map-sequence-id-333602

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  15. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Promoter
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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  19. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  22. pComb3XTT

    Plasmid
    #63891
    Purpose
    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)
    Depositor
    Carlos Barbas
    Article
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Insert
    TT Fab
    Use
    Phage display
    Tags
    6xHis, HA tag, and geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63891-plasmid-map-sequence-id-450336

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  29. pPMVAd3

    Plasmid
    #98298
    Purpose
    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.
    Insert
    PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98298-plasmid-map-sequence-id-190181

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  34. AlkB-D135T

    Plasmid
    #160433
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Threonine; deleted t…
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160433-plasmid-map-sequence-id-318950

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Showing: 1241 - 1280 of 18068 results